Molecular identification of ectomycorrhizal fungi associated with Dipterocarpaceae
Molecular identification of ectomycorrhizal fungi associated with Dipterocarpaceae
复制标题
龙脑香科外生菌根真菌的分子鉴定
作者：
S. Sirikantaramas;N. Sugioka;S. S. Lee;Latiff A. Mohamed;Hua Seng Lee;A. Szmidt;T. Yamazaki
我们利用线粒体大（ML）亚基核糖体DNA序列、核糖体DNA内转录间隔区（ITS）序列、叶绿体DNA的部分matK序列和叶绿体DNA的trnL-trnF间隔区序列鉴定了龙脑香科外生菌根根尖的真菌和植物。我们使用系统发育分析将外生菌根根的 DNA 序列与可用数据库中的序列进行了比较。通过ML和/或ITS序列鉴定的外生菌根真菌属于以下7个科：红菇科、牛肝菌科、Cortinariaceae、Thelephoraceae、Hygrophoraceae、硬皮科、鸡油菌科和伞形毒科。通过部分 matK 和/或 trnL-trnF 序列鉴定的龙脑香科物种属于以下属：Dryobalanops、Hopea、Vatica、Cotylelobium、Anisoptera、Upuna、Dipterocarpus 和 Shorea。从未有报道称，Thelephoraceae 的成员与龙脑香科形成共生根，但它们的数量似乎是最丰富的，并且通常与 Shorea 物种相关。需要对其他龙脑香科物种进行分析来证实这些结果。
We identified both the fungi and plants of ectomycorrhizal root tips of Dipterocarpaceae using the sequence of mitochondrial large (ML) subunit ribosomal DNA, internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA, partial matK sequence of chloroplast DNA and trnL-trnF spacer sequence of chloroplast DNA. We compared the DNA sequences from ectomycorrhizal roots with the sequences from the available databases using phylogenetic analysis. The ectomycorrhizal fungi identified by ML and/or ITS sequences belonged to the following seven families: Russulaceae, Boletaceae, Cortinariaceae, Thelephoraceae, Hygrophoraceae, Sclerodermataceae, Cantharellaceae and Amanitaceae. The Dipterocarpaceae species identified by partial matK and/or trnL-trnF sequences belonged to the following genera: Dryobalanops, Hopea, Vatica, Cotylelobium, Anisoptera, Upuna, Dipterocarpus and Shorea. Members of Thelephoraceae, which have never been reported to form symbiotic roots with Dipterocarpaceae, appeared to be the most abundant and were usually associated with Shorea species. Analyses of other Dipterocarpaceae species are needed to confirm these results.