Primary structure of human indoleamine 2,3-dioxygenase deduced from the nucleotide sequence of its cDNA.
Primary structure of human indoleamine 2,3-dioxygenase deduced from the nucleotide sequence of its cDNA.
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从 cDNA 核苷酸序列推导出人吲哚胺 2,3-双加氧酶的一级结构。
DOI：
10.1093/nar/18.2.367
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发表时间：
1990
影响因子：
14.9
通讯作者：
R. Kido
中科院分区：
文献类型：
--
作者：
S. Toné;O. Takikawa;A. Habara;A. Kadoya;R. Yoshida;R. Kido
吲哚胺2，3-双加氧酶（IDO）是一种色氨酸降解长度，编码一种含有403个残基的蛋白质，具有酶活性，受干扰素（IFN）诱导（1，2）。作为第一步，分子量为45，324。为了阐明IFN诱导IDO的机制，我们在此报道了从cDNA克隆序列推断的人IDO的一级结构。这些克隆是从Xgtl 1文库中分离的，该文库是由IFN-y处理的HEL细胞（Flow 2000）的poly（A）+ RNA与针对IDO的单克隆抗体制备的。预测的氨基酸序列包含纯化的IDO蛋白的内肽序列的部分氨基酸序列（下划线字母）。开放阅读框为1209个核苷酸
Indoleamine 2,3-dioxygenase (IDO), a tryptophan degrading length and encodes a protein containing 403 residues and having enzyme, is induced by interferons (IFNs) (1, 2). As a first step a molecular weight of 45,324. towards elucidating the mechanism of IDO induction by IFN's, we report here the primary structure of human IDO deduced from sequences of cDNA clones. These clones were isolated from a Xgtl 1 library prepared from poly (A) + RNA of IFN-y -treated HEL cells (Flow 2000) with a monoclonal antibody against IDO. The predicted amino acid sequence contained partial amino acid sequences (underlined letters) of endopeptidic digests of purified IDO protein. The open reading frame is 1209 nucleotides in