Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss)
Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1016/j.cbpc.2009.10.009
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发表时间：
2010-03-01
影响因子：
3.9
通讯作者：
Smital, Tvrtko
中科院分区：
文献类型：
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作者：
Loncar, Jovica;Popovic, Marta;Smital, Tvrtko
在这项研究中，我们研究了一系列的ABC外排转运蛋白在各种虹鳟鱼（虹鳟鱼）组织的基因表达。基于其报告的毒理学相关性，我们使用定量真实的时间PCR SYBR绿色定量方法，结合绝对和相对方法，定量来自三个不同家族的八种ABC转运蛋白的RNA表达：abcb 1和abcb 11，abcc 15和abcg 2。mRNA转录水平的测定在七个组织：肝脏，脑，性腺，肾脏，鳃，近端肠和远端肠，并获得的表达谱进行了比较与相关的哺乳动物组织的数据。大多数分析的基因显示出与哺乳动物中发现的基因表达模式相似的基因表达模式，但有一些明显的例外。例如，在一个示例中，鳃在我们的研究中的特征是所有分析的ABC基因的极低表达;尽管ABCC 1（MRP 1）在保护哺乳动物细胞免受化学毒素侵害中具有显著作用，但我们发现这种转运蛋白在鳟鱼组织中的低表达。两者合计，我们的研究提供了第一次深入了解（生态）毒理学相关的ABC转运蛋白的分布模式，作为进一步研究的必要基础，以更好地了解ABC转运蛋白在鱼类中的生理和/或保护作用。(C)2009爱思唯尔公司All rights reserved.
In this study we examined gene expression of a series of ABC efflux transporters in various rainbow trout (Oncorhynchus mykiss) tissues. Based on their reported toxicological relevance, we have used quantitative real time PCR SYBR green quantification methodology, with combination of absolute and relative approach, to quantify RNA expression of eight ABC transporters from three different families: abcb1 and abcb11, abcc15 and abcg2. Level of mRNA transcripts was measured in seven tissues: liver, brain, gonads, kidney, gills, proximal intestine and distal intestine, and the obtained expression profiles were compared with data available for related mammalian tissues. Most of the analyzed genes showed similar gene expression pattern as the ones found in mammals, with some notable exceptions. E.g., gills were in our study characterized by extremely low expression of all analyzed ABC genes; and despite the pronounced role of ABCC1 (MRP1) in protection of mammalian cells from chemical toxins, we found low expression of this transporter in trout tissues. Taken together, our study offers the first thorough insight into distribution pattern of (eco)toxicologically relevant ABC transporters, serving as a necessary base for further studies directed to better understanding of physiological and/or protective role of ABC transporters in fish. (C) 2009 Elsevier Inc. All rights reserved.