Differential expression of candidate genes for lignin biosynthesis under drought stress in maize leaves
Differential expression of candidate genes for lignin biosynthesis under drought stress in maize leaves
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干旱胁迫下玉米叶片木质素生物合成候选基因的差异表达
DOI：
10.1007/bf03195675
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Fu, F-L.
中科院分区：
文献类型：
--
作者：
Hu, Y.;Li, W-Ch.;Fu, F-L.
为了给抗旱性改良分子选择标记的开发提供信息，本研究采用蛋白质定量分析、实时荧光定量PCR和田间评价等方法，对干旱胁迫下玉米候选基因的差异表达进行了鉴定。在第7叶期用聚乙二醇模拟干旱胁迫17、24和48 h，从2个耐旱自交系中采集叶片样品，通过双向电泳和肽质量指纹分析进行蛋白质分析。在500多种蛋白质中，有58种蛋白质是对干旱胁迫的反应。干旱诱导的2506、3507和4506位点分别与肉桂醇脱氢酶、细胞色素蛋白96 A8和S-腺苷-L-甲硫氨酸合酶具有相似性。采用实时荧光定量PCR技术，对干旱胁迫和充分浇水条件下3个耐旱和1个耐旱敏感的自交系中木质素合成关键酶的表达进行了定量分析。在干旱胁迫初期，肉桂醇脱氢酶和咖啡酸O-甲基转移酶的表达量下降，但在干旱胁迫24 h后，这两种酶的表达量在3个耐旱品系中恢复，而在干旱敏感品系中则没有恢复。以6个耐旱性不同的玉米自交系为材料，在干旱胁迫和充分浇水条件下，研究了叶片木质素含量、开花吐丝间隔期和单株粒重的变化。计算了这3个性状的耐旱性系数，并估算了它们之间的相关系数。叶片木质素含量在自交系间存在显著差异，对干旱胁迫的响应也存在显著差异。叶片木质素含量的耐旱系数与单株粒重、开花吐丝间隔期密切相关。这些结果表明，叶片木质素含量是评价玉米耐旱性的一个有用指标。根据候选基因的差异表达，可以开发分子选择标记，用于玉米耐旱性改良。
In order to provide information for the development of molecular selection markers for drought tolerance improvement, the methods of prometric analysis, quantitative real-time PCR and field evaluation were employed for the identification of the differential expression of candidate genes under drought stress in maize. At seventeen, twenty-four and forty-eight hours of polyethylene glycol-simulated drought stress at the seventh leaf stage, leaf samples were collected from two drought-tolerant inbred lines for prometric analysis by two-dimensional electrophoresis and peptide mass fingerprinting. Fifty-eight proteins out of more than 500 were found in response to drought stress. Three drought-induced spots 2506, 3507 and 4506 showed sequence similarity with cinnamyl alcohol dehydrogenase, cytochrome protein 96A8 and S-adenosyl-L-methionine synthase, respectively. The expression of two key enzymes to lignin biosynthesis was quantified by quantitative real-time PCR among three drought-tolerant and one drought-sensitive inbred lines under drought stress and well-watered control conditions. After a decrease at the beginning of drought stress, the expression of cinnamyl alcohol dehydrogenase and caffeateO-methyltransferase recovered at twenty-four hours of the drought stress in the three drought-tolerant lines, but not in the drought-sensitive lines. Leaf lignin content, anthesis-silking interval and grain weight per plant were investigated with six inbred lines of varying drought tolerance under drought stress and well-watered control. Drought tolerance coefficients of these three characters were calculated and the correlation coefficients among these drought tolerance coefficients were estimated. Significant difference in leaf lignin content was found among the inbred lines and in response to drought stress. Close correlations were observed between the drought tolerant coefficients for leaf lignin content and grain weight per plant, and between the drought tolerant coefficients for leaf lignin content and anthesis-silking interval. These results indicate that leaf lignin content is a useful index for evaluation of drought tolerance in maize. Molecular selection markers can be developed on the basis of differential expression of the candidate genes and applied to maize improvement for drought tolerance.