Imbalance of dietary nutrients and the associated differentially expressed genes and pathways may play important roles in juvenile Kashin-Beck disease
Imbalance of dietary nutrients and the associated differentially expressed genes and pathways may play important roles in juvenile Kashin-Beck disease
复制标题
膳食营养失衡以及相关的差异表达基因和通路可能在幼年卡申-贝克病中发挥重要作用
DOI：
10.1016/j.jtemb.2018.01.012
复制
发表时间：
2018-01-01
影响因子：
3.5
通讯作者：
Guo, Xiong
中科院分区：
文献类型：
--
作者：
Ning, Yujie;Wang, Xi;Guo, Xiong
背景：大骨节病（KBD）是我国一种儿童期发病的地方性骨关节病。营养素（包括微量元素）可能在大骨节病的发病中起积极作用。目的：研究大骨节病病区儿童营养素的摄入情况，寻找与营养素失衡相关的基因差异表达。在这项横断面研究中，采用连续3天24 h半定量膳食回顾调查表，用CDGSS 3.0软件估算儿童每日营养素摄入量。应用基因谱分析技术对大骨节病患儿外周血单个核细胞差异表达基因进行分析。GOC、CTD、KEGG和REACTOME数据库用于建立营养素与营养素相关差异表达基因和途径之间的关系。采用SPSS18.0软件进行统计学分析。补硒（Se +）病区未补硒儿童每日硒摄入量显著低于补硒病区（29.3与29.6 mg/d相似）和非流行区（27.8 ± 7.9 mg/d）与非补硒（Se-）大骨节病地区（32.9 ± 7.9 mg/d，c（2）= 20.24，P <0.01）相比。硒+大骨节病病区儿童维生素B-2、维生素C、维生素E、钙、铁、锌、碘等多种营养素摄入不足更为严重。基因图谱分析结合生物信息学技术，筛选出34个营养相关差异表达基因和10条与幼年大骨节病病理变化相关的重要通路。结论：膳食营养失衡及营养相关差异表达基因和通路可能在幼年大骨节病的发生发展中起重要作用。
Background: Kashin-Beck disease (KBD) is a childhood-onset endemic osteoarthropathy in China. Nutrients including trace elements may play active roles in the development of KBD.Objective: This study aimed to estimate the nutrient intakes of children in endemic areas and to identify the imbalanced nutrients associated differentially expressed genes in the juvenile patients with KBD.Methods: In this cross-sectional study, a consecutive 3 day 24 h semi-quantitative dietary retrospect questionnaire was conducted to estimate the daily nutrient intakes of children using CDGSS 3.0 software. Gene profile analysis was employed to identify differentially expressed genes in peripheral blood mononuclear cells of children with KBD. GOC, CTD, KEGG, and REACTOME databases were used to establish the relationship between nutrients and nutrients-associated differentially expressed genes and pathways. Statistical analyses were accomplished by SPSS 18.0 software.Results: Daily Se intakes without supplementation of children were significantly lower in Se-supplemented (Se +) KBD areas (29.3 similar to 29.6 mg/d) and non-endemic area (27.8 +/- 7.9 mg/d) compared to non-Se-supplemented (Se-) KBD area (32.9 +/- 7.9 mg/d, c(2) = 20.24, P < .01). Children in Se + KBD areas were suffering more serious insufficient intake of multiple nutrients, including vitamins-B-2/-C/-E, Ca, Fe, Zn and I. Gene profile analysis combined with bioinformatics technique identified 34 nutrients associated differentially expressed genes and 10 significant pathways which are related to the pathological changes in juvenile KBD.Conclusions: Imbalance of dietary nutrients and nutrients-associated differentially expressed genes and pathways may play important roles in the development of juvenile KBD.