A novel regulation system of gene expression responding to protease signal.
A novel regulation system of gene expression responding to protease signal.
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响应蛋白酶信号的新型基因表达调控系统。
DOI：
10.1093/nass/1.1.149
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Maeda
中科院分区：
文献类型：
--
作者：
S. Sakakihara;K. Fujii;Y. Katayama;M. Maeda
合成了一种阳离子聚合物，其中引入了与Caspase3(CPCC)的切割位点。在Caspase3存在或不存在的情况下，用凝胶电泳法研究了CPCC聚合物与DNA的相互作用。当CPCC聚合物加入到DNA溶液中时，随着聚合物加入量的增加，DNA的迁移受到抑制。这些结果表明，合成的聚合物与DNA发生了相互作用，Caspase3对聚合物侧链的断裂减弱了聚合物与DNA的相互作用。这一系统将有助于设计一种响应细胞凋亡信号的人工基因调控系统。
A cationic polymer, in which a cleavage site with Caspase 3 (CPCC) was introduced, was synthesized. Then an interaction between the polymer and a DNA was investigated with gel electrophoresis in the presence or absence of Caspase 3. When the CPCC polymer was added to the DNA solution, the migration of the DNA was suppressed with the increase of the addition of the polymer in the gel electrophoresis. However, this delay was cancelled with the addition of Caspase 3. These results suggested that this synthesized polymer interacted with the DNA, and the side chain cleavage of this polymer by Caspase 3 reduced the interaction between the polymer and the DNA. Such system would be useful for the design of an artificial gene regulation system responding to cellular apoptotic signal.