Matching the Diversity of Sulfated Biomolecules: Creation of a Classification Database for Sulfatases Reflecting Their Substrate Specificity.
Matching the Diversity of Sulfated Biomolecules: Creation of a Classification Database for Sulfatases Reflecting Their Substrate Specificity.
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DOI：
10.1371/journal.pone.0164846
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Michel G
中科院分区：
文献类型：
--
作者：
Barbeyron T;Brillet-Guéguen L;Carré W;Carrière C;Caron C;Czjzek M;Hoebeke M;Michel G
硫酸酯酶从各种分子上裂解硫酸酯基团，构成生物学和工业上重要的一类酶。然而，与硫酸化化合物的巨大多样性相比，其底物已被表征的硫酸化酶的数量是有限的，产生硫酸化酶的功能注释特别容易出现缺陷和误解。在基因组数据爆炸的背景下，硫酸酯酶迫切需要一个分类系统，可以更好地预测底物特异性和设置功能注释的限制。在这里，在综述了硫酸化化合物的多样性和已知的硫酸酯酶之后，我们提出了一个基于序列同源性的分类数据库SulfAtlas（http：//abims.sb-roscoff.fr/sulfatlas/），该数据库由四个硫酸酯酶家族组成。的甲酰甘氨酸依赖性硫酸酯酶，构成最大的家庭，也分为73个亚家族，每个亚家族对应于一个已知的特异性或一个未知的底物的系统发育方法。SulfAtlas总结了有关硫酸酯酶不同家族的信息。在族内，网页显示其子族（如果存在）的列表和EC编号的列表。家族或亚家族页面显示了一些描述符和一个表格，其中包含链接到数据库UniProt、ExplorEnz和PDB的所有UniProt登录号。
Sulfatases cleave sulfate groups from various molecules and constitute a biologically and industrially important group of enzymes. However, the number of sulfatases whose substrate has been characterized is limited in comparison to the huge diversity of sulfated compounds, yielding functional annotations of sulfatases particularly prone to flaws and misinterpretations. In the context of the explosion of genomic data, a classification system allowing a better prediction of substrate specificity and for setting the limit of functional annotations is urgently needed for sulfatases. Here, after an overview on the diversity of sulfated compounds and on the known sulfatases, we propose a classification database, SulfAtlas (http://abims.sb-roscoff.fr/sulfatlas/), based on sequence homology and composed of four families of sulfatases. The formylglycine-dependent sulfatases, which constitute the largest family, are also divided by phylogenetic approach into 73 subfamilies, each subfamily corresponding to either a known specificity or to an uncharacterized substrate. SulfAtlas summarizes information about the different families of sulfatases. Within a family a web page displays the list of its subfamilies (when they exist) and the list of EC numbers. The family or subfamily page shows some descriptors and a table with all the UniProt accession numbers linked to the databases UniProt, ExplorEnz, and PDB.
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影响因子：
3.4
作者：
Aspeborg H;Coutinho PM;Wang Y;Brumer H 3rd;Henrissat B
通讯作者：
Henrissat B
影响因子：
5.7
作者：
Bond, CS;Clements, PR;Guss, JM
通讯作者：
Guss, JM
影响因子：
4.8
作者：
Eichhorn, E;vanderPloeg, JR;Leisinger, T
通讯作者：
Leisinger, T
影响因子：
4.8
作者：
Dotson, SB;Smith, CE;Kishore, GM
通讯作者：
Kishore, GM
影响因子：
3
作者：
Campanella, JJ;Bitincka, L;Smalley, J
通讯作者：
Smalley, J