NATURAL CYCLOHEXIMIDE RESISTANCE IN YEAST - THE ROLE OF RIBOSOMAL-PROTEIN L41
NATURAL CYCLOHEXIMIDE RESISTANCE IN YEAST - THE ROLE OF RIBOSOMAL-PROTEIN L41
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DOI：
10.1111/j.1432-1033.1993.tb17827.x
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发表时间：
1993-04-15
期刊：
影响因子：
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通讯作者：
CANNON, M
中科院分区：
文献类型：
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作者：
DEHOUX, P;DAVIES, J;CANNON, M
乳酸克鲁维酵母对高浓度（1 mg/ml）的抗生素放线菌酮具有抗性。使用体外翻译研究，证实了这种极端抗性是核糖体的特性。K. lactis克隆到酿酒酵母中。对该决定簇的核苷酸序列分析表明，抗性是由K.乳核糖体蛋白L41。K. lactis中仅含有一个编码该蛋白的基因拷贝，该基因位于染色体III。相反。S.发现酿酒酵母含有对应核糖体蛋白L41的基因的多个拷贝，其定位于三条染色体V、XIV和VIII中的两条。由于K. lactis引起基本上完全的保护以抵抗药物的抑制作用，它可能在真核基因转移研究中作为选择性标记物特别有用。
The yeast Kluyveromyces lactis is resistant to high concentrations (1 mg/ml) of the antibiotic cycloheximide. Using in vitro translation studies it was confirmed that this extreme resistance is a property of ribosomes. The resistance determinant from K. lactis was cloned into Saccharomyces cerevisiae. Nucleotide sequence analysis of the determinant demonstrated that resistance was conferred by the K. lactis ribosomal protein L41. K. lactis was shown to contain only one copy of the gene that encodes this protein and the gene was located to chromosome III. In contrast. S. cerevisiae was found to contain multiple copies of the gene for the corresponding ribosomal protein L41 which mapped to two of the three chromosomes V, XIV and VIII. Since the cycloheximide-resistance gene of K. lactis causes essentially complete protection against inhibition by the drug it is likely to be particularly useful as a selective marker in eukaryotic gene transfer studies.