Xanthene Dyes Induce Membrane Permeabilization of Bacteria and Erythrocytes by Photoinactivation
Xanthene Dyes Induce Membrane Permeabilization of Bacteria and Erythrocytes by Photoinactivation
复制标题
DOI：
10.1111/j.1751-1097.2012.01080.x
复制
发表时间：
2012-03-01
影响因子：
3.3
通讯作者：
Katsu, Takashi
中科院分区：
文献类型：
--
作者：
Kato, Hisato;Komagoe, Keiko;Katsu, Takashi
我们分析了氧杂蒽染料引起的细菌和红细胞膜功能的光失活。测试的染料是玫瑰红、荧光桃红B、赤藓红B和曙红B。这些染料在光照射的几分钟内诱导K+从金黄色葡萄球菌细胞中泄漏，顺序为玫瑰红>荧光桃红B >赤藓红B >曙红B。染料抑制呼吸的能力很弱，除了玫瑰红，染料消散在类似的时间痕迹与K+渗透性的变化膜电位。氧杂蒽染料也诱导K+从牛红细胞的光照射后，在相同的顺序观察到与细菌的泄漏。此外，我们发现，导致K+从红细胞泄漏的能力与染料诱导的形态学变化，形成一个圆齿状的形式从正常的盘状。这些结果进行了讨论与能力的吨染料产生单线态氧和细菌细胞的结合，并进一步比较与阳离子卟啉，诱导光灭活的细菌通过呼吸抑制的行动。
We analyzed the photoinactivation of the membrane functions of bacteria and erythrocytes induced by xanthene dyes. The dyes tested were rose bengal, phloxine B, erythrosine B and eosin B. These dyes induced the leakage of K+ from Staphylococcus aureus cells within minutes of photoirradiation, in the order of rose bengal > phloxine B > erythrosine B > eosin B. The ability of dyes to inhibit respiration was weak, except for rose bengal, and the dyes dissipated the membrane potential in similar time traces with changes in K+ permeability. The xanthene dyes also induced the leakage of K+ from bovine erythrocytes upon photoirradiation in the same order as that observed with bacteria. Furthermore, we found that the ability to cause the leakage of K+ from erythrocytes was associated with dye-induced morphological changes, forming a crenated form from the normal discoid. These results are discussed in connection with the ability of xanthene dyes to generate singlet oxygen and bind to bacterial cells, and further compared with the actions of cationic porphyrins, which induced photoinactivation of bacteria through respiratory inhibition.