SAGE identification of gene transcripts with profiles unique to pluripotent mouse R1 embryonic stem cells
SAGE identification of gene transcripts with profiles unique to pluripotent mouse R1 embryonic stem cells
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DOI：
10.1006/geno.2002.6687
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发表时间：
2002-02-01
期刊：
影响因子：
4.4
通讯作者：
Boheler, KR
中科院分区：
文献类型：
--
作者：
Anisimov, SV;Tarasov, KV;Boheler, KR
识别调节多能性和自我更新的信号是理解干细胞生物学的基础。为了量化多能R1胚胎干细胞(ES)的功能活性基因组，我们使用基因表达序列分析(SAGE)的方法对总共140,313个SAGE标签进行了测序。在44,569个独特转录本中，9%与非冗余GenBank数据库中的已知基因匹配，而>；35%的独特标签与任何已知的小鼠序列都不匹配。ES细胞SAGE目录中相对丰富(大于或等于20)的标签与公开可获得的SAGE数据集的比较确定了16个转录本，其丰度分布是多能R1 ES细胞所独有的。我们通过RTPCR确认了12个基因，包括转录因子KLF2、下丘脑神经激素甘丙素、促凋亡信号因子Bax以及细胞周期进展相关蛋白CDK4和PAL31。这项研究的数据为干细胞的详细转录组分析提供了一个起点。
The identification of signals that regulate pluripotentiality and self-renewal is fundamental to the understanding of stem cell biology. To quantify the functionally active genome of pluripotent R1 embryonic stem (ES) cells, we used the method of serial analysis of gene expression (SAGE) to sequence a total of 140,313 SAGE tags. Of 44,569 unique transcripts, 9% matched known genes in the nonredundant GenBank database, whereas > 35% of the unique tags did not match any known mouse sequence. Comparisons of relatively abundant (greater than or equal to 20) tags in the ES cell SAGE catalog with publicly available SAGE data sets identified 16 transcripts with an abundance profile unique to pluripotent R1 ES cells. We confirmed 12 by RTPCR including those encoding KLF2, a transcription factor; galanin, a hypothalamic neurohormone; BAX, a proapoptotic signaling factor; and CDK4 and PAL31, cell cycle progression associated proteins. The data from this study provide a starting point for detailed transcriptome analyses of stem cells.