Cytotoxicity evaluation of dental resin composites and their flowable derivatives
Cytotoxicity evaluation of dental resin composites and their flowable derivatives
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DOI：
10.1007/s00784-004-0293-0
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发表时间：
2005-03-01
影响因子：
3.4
通讯作者：
Milhem, MM
中科院分区：
文献类型：
--
作者：
Al-Hiyasat, AS;Darmani, H;Milhem, MM
组分从牙科复合材料释放到周围组织中可引起不良组织反应。因此，本研究调查了三种类型的牙科复合材料的细胞毒性与他们的流动性衍生物，并确定从这些材料中释放的化合物，通过高效液相色谱法（HPLC）分析。将来自每种复合材料（Admira、Z250、Tetric Ceram）的15个样品及其15个可流动物（Admira Flow、Tetric Flow、Feltik Flow）以圆盘形式制备，并将每种材料分成两组，每组10个和5个。第一组（10个圆盘）用于通过测量相对于Teflon对照的细胞代谢活性（3 {4，5-二甲基噻唑-2-基}-2，5-二苯基溴化四唑[MTT]测定）来评价材料对balb/c 3 T3成纤维细胞的细胞毒性，而第二组（5个圆盘）用于通过HPLC分析来确定从每种材料沥滤到培养基中的组分。结果表明，Z250和Tetric Ceram的细胞毒性低于其可流动衍生物。然而，赋形剂Admira的细胞毒性显著高于Admira Flow。在标准复合材料中，Tetric Ceram的细胞毒性最小，Admira最大。此外，在所测试的可流动材料中，Tetric flow的细胞毒性最大，Admira flow的细胞毒性最小。HPLC分析显示，所有材料的洗脱液中均存在双酚A甘油二甲基丙烯酸酯（bis-GMA）和三甘醇二甲基丙烯酸酯（TEGDMA），而除Feltik Flow外，所有洗脱液中均存在氨基甲酸酯二甲基丙烯酸酯（UDMA）。总之，可流动衍生物的细胞毒性高于传统复合材料，而ormocer Admira Flow的细胞毒性低于Admira复合材料。
The release of components from dental composite into surrounding tissue may cause an adverse tissue reaction. Thus, this study investigated the cytotoxicity of three types of dental composites with their flowable derivatives and determined the compounds released from these materials by high-performance liquid chromatography (HPLC) analysis. Fifteen specimens from each composite (Admira, Z250, Tetric Ceram) with fifteen of their flowables (Admira Flow, Tetric Flow, Feltik Flow) were prepared in the form of discs and divided into two groups of 10 and 5 for each material. The first group (10 discs) was used to evaluate the cytotoxicity of the material on balb/c 3T3 fibroblasts by measuring cellular metabolic activity (3 {4,5-dimethylthiazol-2-yl}-2,5-diphenyltetrazolium bromide [MTT] assay) relative to Teflon controls, while the second group (5 discs) was used to determine the leached components from each material into culture medium by HPLC analysis. The results revealed that Z250 and Tetric Ceram were less cytotoxic than their flowable derivatives. However, the ormocer, Admira, was significantly more cytotoxic than Admira Flow. Among the standard composites, Tetric Ceram was the least cytotoxic and Admira the most. Furthermore, Tetric flow was the most cytotoxic and Admira flow was significantly the least cytotoxic among the flowable materials tested. HPLC analysis revealed hisphenol A glycerolate dimethacrylate (bis-GMA) and triethylene glycol dimethacrylate (TEGDMA) in the eluates of all the materials, while urethane dimethacrylate (UDMA) was present in all eluates except that of Feltik Flow. In conclusion, the flowable derivatives are more cytotoxic than the traditional composites whereas the ormocer Admira Flow is less cytotoxic than the Admira composite.