Bioactive Diterpenoids from the Stems of Euphorbia royleana.
Bioactive Diterpenoids from the Stems of Euphorbia royleana.
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DOI：
10.1021/acs.jnatprod.8b00493
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发表时间：
2019-02
影响因子：
5.1
通讯作者：
Peixia Wang;C. Xie;Lijun An;Xueyuan Yang;Yaru Xi;Shuo Yuan;Chenyue Zhang;Muhetaer Tuerhong;Da-qing Jin;Dongho Lee;Jie Zhang;Y. Ohizumi;Jing Xu;Yuanqiang Guo
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文献类型：
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作者：
Peixia Wang;C. Xie;Lijun An;Xueyuan Yang;Yaru Xi;Shuo Yuan;Chenyue Zhang;Muhetaer Tuerhong;Da-qing Jin;Dongho Lee;Jie Zhang;Y. Ohizumi;Jing Xu;Yuanqiang Guo
本文报道了从滇大戟（Euphorbia royleana）茎的甲醇提取物中分离得到的两个二萜烷（1和2）、两个对映阿替烷（3和4）、两个对映贝壳杉烷（5和6）、两个对映松香烷（7和8）和一个对映异海松烷（9）型二萜和12个已知的类似物。通过广泛的光谱方法和ECD数据分析确定了它们的结构，包括绝对构型。在脂多糖刺激的BV-2细胞中生物学地检测这些二萜类化合物的一氧化氮抑制活性，其中化合物1、2、5-7、10和12具有低于40 μM的IC 50值。利用分子对接技术研究了化合物1、2、5-7、10和12的可能作用机制。
Two ingenane- (1 and 2), two ent-atisane- (3 and 4), two ent-kaurane- (5 and 6), two ent-abietane- (7 and 8), and one ent-isopimarane-type (9) diterpenoid and 12 known analogues have been isolated from the methanolic extract of the stems of Euphorbia royleana. Their structures, including absolute configurations, were determined by extensive spectroscopic methods and ECD data analysis. The nitric oxide inhibitory activities of those diterpenoids were examined biologically in lipopolysaccharide-stimulated BV-2 cells, with compounds 1, 2, 5-7, 10, and 12 having IC50 values lower than 40 μM. Molecular docking was used to investigated the possible mechanism of compounds 1, 2, 5-7, 10, and 12.