Nanoscale Covalent Organic Framework for Combinatorial Antitumor Photodynamic and Photothermal Therapy
Nanoscale Covalent Organic Framework for Combinatorial Antitumor Photodynamic and Photothermal Therapy
复制标题
用于组合抗肿瘤光动力和光热疗法的纳米级共价有机框架
DOI：
10.1021/acsnano.9b06467
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发表时间：
2019-11-01
期刊：
影响因子：
17.1
通讯作者：
Dong, Yu-Bin
中科院分区：
文献类型：
--
作者：
Guan, Qun;Zhou, Le-Le;Dong, Yu-Bin
尽管有机分子光敏剂（ps）和光热剂（pta）如卟啉和萘菁具有优异的光动力学和光热性能，但其水溶性差严重阻碍了其生物应用。共价有机骨架（COFs）作为一类新兴的有机晶体多孔材料，具有游离的活性端基（键合缺陷）和较大的内部孔隙，是通过键合缺陷功能化（BDF）和客体包封方式装载疏水有机分子PSs和PTAs的理想纳米载体，可获得用于PDT/PTT联合治疗的多功能纳米药物。在这项工作中，我们报告了在环境条件下通过简易合成方法制备的纳米级COF （nof）。此外，通过逐步BDF和客体包封工艺，成功制备了双峰PDT/PTT治疗纳米剂VONc@COF-Por(3)。共价接枝的卟啉PS （Por）和非共价负载的萘菁PTA （VONc）分别负责纳米试剂的PDT和PTT功能。在可见光（红色LED）和近红外（808 nm激光）照射下，VONc@COF-Por(3)显示出高的O-1(2)生成和光热转换能力（55.9%），从而提供了良好的PDT/PTT联合治疗效果，抑制MCF-7肿瘤细胞的增殖和转移，这在体外和体内实验中都得到了很好的证明。我们相信，本文的研究结果可以显著促进基于ncof的多功能纳米药物在生物医学领域的应用。
Despite the excellent photodynamic and photo thermal properties of organic molecular photosensitizers (PSs) and photothermal agents (PTAs), such as porphyrin and naphthalocyanine, their poor water solubility severely impedes their biological applications. Covalent organic frameworks (COFs), as an emerging class of organic crystalline porous materials, possess free active end groups (bonding defects) and large inner pores, which make them an ideal type of nanocarriers for loading hydrophobic organic molecular PSs and PTAs by both bonding defect functionalization (BDF) and guest encapsulation approaches to obtain multifunctional nanomedicines for PDT/PTT combination therapy. In this work, we report a nanoscale COF (NCOF) prepared via a facile synthetic approach under ambient conditions. Furthermore, a dual-modal PDT/PTT therapeutic nanoagent, VONc@COF-Por (3), is successfully fabricated by stepwise BDF and guest encapsulation processes. The covalently grafted porphyrinic PS (Por) and the noncovalently loaded naphthalocyanine PTA (VONc) are independently responsible for the PDT and PTT functionalities of the nanoagent. Upon visible (red LED) and NIR (808 nm laser) irradiation, VONc@COF-Por (3) displayed high O-1(2) generation and photothermal conversion ability (55.9%), consequently providing an excellent combined PDT/PTT therapeutic effect on inhibiting MCF-7 tumor cell proliferation and metastasis, which was well evidenced by in vitro and in vivo experiments. We believe that the results obtained herein can significantly promote the development of NCOF-based multifunctional nanomedicines for biomedical applications.