Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes1[OPEN]
Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes1[OPEN]
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DOI：
10.1104/pp.15.00155
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发表时间：
2015-06
期刊：
影响因子：
7.4
通讯作者：
Aurine Verkest;M. Byzova;Cindy Martens;P. Willems;T. Verwulgen;Bram Slabbinck;D. Rombaut;Jan Van de Velde;K. Vandepoele;E. Standaert;Marrit Peeters;M. Van Lijsebettens;F. Van Breusegem;M. De Block
中科院分区：
文献类型：
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作者：
Aurine Verkest;M. Byzova;Cindy Martens;P. Willems;T. Verwulgen;Bram Slabbinck;D. Rombaut;Jan Van de Velde;K. Vandepoele;E. Standaert;Marrit Peeters;M. Van Lijsebettens;F. Van Breusegem;M. De Block
对卡诺拉油菜的能量利用效率和耐旱性的选择导致具有不同转录组和染色质修饰谱的稳定epilines，支持它们的上级性能。为了提高作物的产量潜力和稳定性，采用了主要具有直接遗传基础的综合育种策略，但表观遗传学在改善复杂性状方面的效用尚不清楚。更好地了解表观基因组的地位及其对农艺性状的贡献将有助于开发将复杂性状的表观遗传成分纳入育种计划的方法。从等基因油菜（甘蓝型油菜）系开始，通过重复选择三代来产生epilines，以提高能量利用效率和耐旱性。这些epilines具有增强的能量利用效率，耐旱性和氮利用效率。转录组分析的epilines和一个线选择其能源利用效率只揭示了共同的差异表达的基因有关的发病压力耐受调节信号传导事件。盐，渗透，脱落酸和干旱处理的反应相关的基因特异性差异表达的耐旱epilines。表观基因组的状态，评分为组蛋白3的赖氨酸-4的差异三甲基化，通过靶向干旱响应基因和促进差异表达基因的转录进一步支持表型。从这些结果中，我们得出结论，油菜表观基因组可以通过选择来塑造，以提高能量利用效率和胁迫耐受性。因此，这些发现保证了将表观遗传学纳入育种策略的进一步发展。
Selection for both energy use efficiency and drought tolerance in canola leads to stable epilines with distinct transcriptome and chromatin modification profiles, supporting their superior performance. To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would help in developing approaches to incorporate the epigenetic component of complex traits into breeding programs. Starting from isogenic canola (Brassica napus) lines, epilines were generated by selecting, repeatedly for three generations, for increased energy use efficiency and drought tolerance. These epilines had an enhanced energy use efficiency, drought tolerance, and nitrogen use efficiency. Transcriptome analysis of the epilines and a line selected for its energy use efficiency solely revealed common differentially expressed genes related to the onset of stress tolerance-regulating signaling events. Genes related to responses to salt, osmotic, abscisic acid, and drought treatments were specifically differentially expressed in the drought-tolerant epilines. The status of the epigenome, scored as differential trimethylation of lysine-4 of histone 3, further supported the phenotype by targeting drought-responsive genes and facilitating the transcription of the differentially expressed genes. From these results, we conclude that the canola epigenome can be shaped by selection to increase energy use efficiency and stress tolerance. Hence, these findings warrant the further development of strategies to incorporate epigenetics into breeding.