Excess Polymorphisms in genes for membrane proteins in Plasmodium falciparum
Excess Polymorphisms in genes for membrane proteins in Plasmodium falciparum
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DOI：
10.1126/science.1075642
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发表时间：
2002-10-04
期刊：
影响因子：
56.9
通讯作者：
Winzeler, EA
中科院分区：
文献类型：
--
作者：
Volkman, SK;Hartl, DL;Winzeler, EA
The detection of single-nucleotide polymorphisms in pathogenic microorganisms has normally been carried out by trial and error. Here we show that DNA hybridization with high-density oligonucleotide arrays provides rapid and convenient detection of single-nucleotide polymorphisms in Plasmodium falciparum, despite its exceptionally high adenine-thymine (AT) content (82%). A disproportionate number of polymorphisms are found in genes encoding proteins associated with the cell membrane. These genes are targets for only 22% of the oligonucleotide probes but account for 69% of the polymorphisms. Genetic variation is also enriched in subtelomeric regions, which account for 22% of the chromosome but 76% of the polymorphisms.
