DNA sequences encoding enolase are remarkably conserved from yeast to mammals.
DNA sequences encoding enolase are remarkably conserved from yeast to mammals.
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DOI：
10.1016/0024-3205(94)00534-6
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发表时间：
1994
期刊：
影响因子：
6.1
通讯作者：
M. Verma;S. Dutta
中科院分区：
文献类型：
--
作者：
M. Verma;S. Dutta
烯醇化酶（2-磷酸-D-甘油酸水解酶，EC 4.2.1.11），特别是同种型神经元特异性烯醇化酶（NSE），主要定位于神经元和神经内分泌细胞，是脑肿瘤的癌症诊断标志物。使用杂交技术、序列差异百分比和氨基酸分析，研究了来自人、狗、牛、大鼠、小鼠、兔、鸡和酵母细胞的烯醇化酶编码 DNA 序列的同源性。由于烯醇酶是糖酵解途径中的一种重要酶，因此迄今为止在所有测试的生物体中都发现了烯醇酶编码 DNA 序列。人们发现，人类的酶在结构上更像猴子和狗的酶，而不是鸡和酵母的酶。讨论了烯醇化酶编码 DNA 序列遗传保守性的存在对于理解协同进化以及分化过程中转录后调控的影响。这是第一份关于在多种生物体中确定烯醇酶编码区序列差异的报告。
Enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11), particularly isoform neuron-specific enolase (NSE), is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. Homology of enolase-coding DNA sequences from human, dog, cow, rat, mouse, rabbit, chicken, and yeast cells was investigated using hybridization techniques, percent sequence divergence, and amino acid analysis. Because enolase is a significant enzyme of the glycolytic pathway, enolase-coding DNA sequences have been found in all organisms tested so far. The human enzyme was found to be more like those of monkey and dog in structure than to those of chicken and yeast. The implications of the existence of the genetic conservation of enolase-coding DNA sequences in understanding concerted evolution as well as post-transcriptional regulation during differentiation are discussed. This is the first report is which sequence divergence in the coding region for enolase has been determined in a variety of organisms.