Gastric cancers overexpress S100A calcium-binding proteins.
Gastric cancers overexpress S100A calcium-binding proteins.
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发表时间：
2002-12
期刊：
影响因子：
11.2
通讯作者：
W. El‐Rifai;C. Moskaluk;M. K. Abdrabbo;Jeffery C. Harper;C. Yoshida;G. Riggins;H. Frierson;S. Powe
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文献类型：
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作者：
W. El‐Rifai;C. Moskaluk;M. K. Abdrabbo;Jeffery C. Harper;C. Yoshida;G. Riggins;H. Frierson;S. Powe
基因表达的系列分析提供了给定细胞群中的定量和全面的表达谱。在我们努力定义胃癌中过度表达的基因时，我们对解剖的肿瘤和正常胃上皮进行了基因表达分析的系列分析。我们鉴定了 91,334 个表达标签，其中 26,633 个是独特的。与正常胃上皮相比，胃癌 (GC) 中表达最上调的 20 个基因 (P < 0.01) 包括几种上皮细胞特异性的角蛋白，如角蛋白 6A、13 和 17。有趣的是，5 种钙结合蛋白（S100A2、S100A7、S100A8、S100A9 和 S100A10）过度表达。对原代 GC 样本进行的定量实时 PCR 表明，20 个肿瘤中有 18 个肿瘤 (90%) 存在 S100A2 过度表达。我们研究的 25-45% GC 样品中其他钙结合蛋白过度表达。我们的结果表明 S100A 蛋白可能对胃肿瘤发生很重要。需要进一步的研究来阐明钙结合蛋白在癌症中的生物学作用。
Serial analysis of gene expression provides quantitative and comprehensive expression profiling in a given cell population. In our efforts to define the genes overexpressed in carcinoma of the stomach, we performed serial analysis of gene expression analyses on dissected neoplastic and normal gastric epithelia. We identified 91,334 expressed tags, including 26,633 that were unique. The 20 most up-regulated genes (P < 0.01) in gastric cancer (GC) compared with normal gastric epithelia included several keratins that are specific for epithelial cells such as keratin 6A, 13, and 17. Interestingly, five calcium-binding proteins (S100A2, S100A7, S100A8, S100A9, and S100A10) were overexpressed. Quantitative real-time PCR on primary GC samples demonstrated overexpression of S100A2 in 18 of 20 tumors (90%). The other calcium-binding proteins were overexpressed in 25-45% of the GC samples that we studied. Our results indicate that S100A proteins may be important for gastric tumorigenesis. Additional investigations are required to elucidate the biological role of calcium-binding proteins in cancer.