Unusual outbreak of post-weaning porcine diarrhea caused by single and mixed infections of rotavirus groups A, B, C, and H
Unusual outbreak of post-weaning porcine diarrhea caused by single and mixed infections of rotavirus groups A, B, C, and H
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DOI：
10.1016/j.vetmic.2016.08.014
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发表时间：
2016-09-25
影响因子：
3.3
通讯作者：
Alfieri, Amauri Alcindo
中科院分区：
文献类型：
--
作者：
Domingues Molinari, Bruna Leticia;Possatti, Flavia;Alfieri, Amauri Alcindo
轮状病毒（RV）是人类和动物严重腹泻的主要原因。9个RV组中的5个（RVA、RVB、RVC、RVE和RVH）先前已在猪中检测到;然而，在全球猪群中，大多数研究强调了由RVA引起的腹泻暴发。在本研究中，我们描述了在断奶后腹泻爆发期间，从单一和混合感染猪的粪便样本中检测和表征RV组A、B、C和H。暴发发生在常规接种用于新生儿腹泻控制的灭活商业疫苗（包括RVA OSU（G5 P [7]）毒株）的单个猪群中。RVC（78%）是在单一（34%）和混合（44%）感染中发现的最常见的组，其次是RVA（46%），RVB（32%）和RVH（18%）。三种RVA菌株的系统发育分析允许表征由G5 P [13]和G9 P [23]代表的两种不同的G/P基因型，其不同于疫苗中发现的G5 P [7]。无论RV组如何，混合感染（54%）比单一感染更普遍。RVB或RVH的检测与其他RV组的存在有关，表明这些RV组在报告的暴发中的继发作用。在同一猪群中检测到RV B、C和H群，表明这些RV是腹泻的病原体，应纳入猪肠道疾病的诊断试验中。这些数据提供了关于RV多样性的新的流行病学信息，需要在未来的研究中加以解决，以更好地了解和预防RV感染。(C)© 2016 Elsevier B. V.版权所有。
Rotaviruses (RVs) are a major cause of severe diarrhea in humans and animals. Five of the nine RV groups (RVA, RVB, RVC, RVE, and RVH) have been previously detected in pigs; however, in pig herds worldwide, most studies highlight diarrhea outbreaks caused by RVA. In the present study, we describe detection and characterization of RV groups A, B, C, and H in fecal samples from pigs with single and mixed infections during a post-weaning diarrhea outbreak. The outbreak occurred in a single pig herd routinely vaccinated with an inactivated commercial vaccine for neonatal diarrhea control that included the RVA OSU (G5P[7]) strain. RVC (78%) was the most prevalent group found in single (34%) and mixed (44%) infections, followed by RVA (46%), RVB (32%), and RVH (18%). Phylogenetic analysis of three RVA strains allowed the characterization of two distinct G/P genotypes represented by G5P[13] and G9P[23], different from G5P [7] found in vaccines. Regardless of the RV group, mixed infections (54%) were more prevalent than single infections. Detection of RVB or RVH was associated with the presence of other RV groups, suggesting a secondary action of these RV groups in the reported outbreak. The detection of RV groups B, C, and H in the same pig herd suggests that these RVs act as causative agents of diarrhea and should be included in the diagnostic tests of porcine enteric diseases. These data provide new epidemiological information on RV diversity that need to be addressed in future studies for a better understanding and prevention of RV infections. (C) 2016 Elsevier B.V. All rights reserved.