Modified Technique for Coronary Artery Ligation in Mice
Modified Technique for Coronary Artery Ligation in Mice
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DOI：
10.3791/3093
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发表时间：
2013-03-01
影响因子：
1.2
通讯作者：
Omerovic, Elmir
中科院分区：
文献类型：
--
作者：
Shao, Yangzhen;Redfors, Bjoen;Omerovic, Elmir
心肌梗死（MI）是人类最重要的死亡原因之一（1-3）。为了提高心肌梗死患者的发病率和死亡率，我们需要更好地了解心肌缺血的病理生理。这些知识可能对确定创新心血管疗法的新治疗靶点有价值(4)。实验性小鼠心肌梗死模型是临床前研究中越来越流行的一种小动物模型，通过永久或暂时结扎左冠状动脉（LCA）诱导心肌梗死(5)。在这个视频中，我们描述了如何一步一步地诱导小鼠实验性心肌梗死的方法。先用2%异氟醚麻醉动物。然后将失去知觉的老鼠插管并连接到呼吸机进行人工通气。左胸刮毛，沿腋中线在皮肤上做1.5 cm切口。左胸大肌直接游离，直到肋骨暴露。将肌肉层拉到一边，用眼睑牵开器固定。在这些准备之后，在第三和第四肋骨之间进行左开胸术，以便看到心脏和左肺的前表面。然后用7-0乙隆缝线结扎LCA动脉近段，通常会导致梗死面积与左心室的40%相似。最后闭胸，动物术后镇痛（Temgesic, 0.3 mg/50 ml, ip）。这些动物被关在一个温暖的笼子里，直到自然恢复。
Myocardial infarction (MI) is one of the most important causes of mortality in humans(1-3). In order to improve morbidity and mortality in patients with MI we need better knowledge about pathophysiology of myocardial ischemia. This knowledge may be valuable to define new therapeutic targets for innovative cardiovascular therapies(4). Experimental MI model in mice is an increasingly popular small-animal model in preclinical research in which MI is induced by means of permanent or temporary ligation of left coronary artery (LCA)(5). In this video, we describe the step-by-step method of how to induce experimental MI in mice.The animal is first anesthetized with 2% isoflurane. The unconscious mouse is then intubated and connected to a ventilator for artificial ventilation. The left chest is shaved and 1.5 cm incision along mid-axillary line is made in the skin. The left pectoralis major muscle is bluntly dissociated until the ribs are exposed. The muscle layers are pulled aside and fixed with an eyelid-retractor. After these preparations, left thoracotomy is performed between the third and fourth ribs in order to visualize the anterior surface of the heart and left lung. The proximal segment of LCA artery is then ligated with a 7-0 ethilon suture which typically induces an infarct size similar to 40% of left ventricle. At the end, the chest is closed and the animals receive postoperative analgesia (Temgesic, 0.3 mg/50 ml, ip). The animals are kept in a warm cage until spontaneous recovery.