Protective effects of recombinant human granulocyte colony stimulating factor in a rat model of necrotizing enterocolitis
Protective effects of recombinant human granulocyte colony stimulating factor in a rat model of necrotizing enterocolitis
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DOI：
10.1007/s00383-006-1728-2
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发表时间：
2006-09-01
影响因子：
1.8
通讯作者：
Korkmaz, Ayse
中科院分区：
文献类型：
--
作者：
Canpolat, Fuat Emre;Yurdakoek, Murat;Korkmaz, Ayse
细胞因子和生长因子在新生儿坏死性小肠结肠炎（NEC）病理生理中的作用尚未明确。本研究旨在观察重组人粒细胞集落刺激因子（G-CSF）对缺氧性NEC大鼠肠上皮细胞的影响。该研究在Sprague道利大鼠幼仔上进行。第1组（未处理，n = 7）大鼠进行缺氧-复氧（H/O），然后返回到标准条件。第2组（G-CSF处理，n = 7）大鼠经受H/O，然后用G-CSF（100 μ g/kg肠内）处理5天。第3组为非缺氧对照组。在第5天处死所有动物，并对肠样品进行组织学检查。对照组无组织病理学改变。未给药大鼠的组织学结果与新生NEC中观察到的结果相似，绒毛和隐窝破坏并延伸至肌层。2组肠道损伤较轻，组织学改变优于1组，但优于3组。第1组的平均组织学分级为2.4（范围2-3），第2组的平均组织学分级为1.2（范围0-2）。两组比较有显著性差异（P < 0.05）。在NEC的实验模型中，G-CSF可以对肠损伤具有保护作用。
The role of cytokines and growth factors in the pathophysiology of neonatal necrotizing enterocolitis (NEC) is not defined clearly yet. The aim of this study was to determine the effects of recombinant human granulocyte colony stimulating factor (G-CSF) on intestinal cells in hypoxia-induced experimental NEC in rats. The study was experimented on Sprague Dawley rat pups. Group 1 (untreated, n = 7) rats were subjected to hypoxia-reoxygenation (H/O) and then were returned to standard conditions. Group 2 (G-CSF treated, n = 7) rats were subjected to H/O, and then were treated with G-CSF (100 mu g/kg enterally) for 5 days. Group 3 was served as nonhypoxic controls. All animals were killed on day five, and histological examination was performed on intestinal samples. There were no histopathological changes in the control group. The histological findings in untreated rats were similar to those seen in neonatal NEC, with destruction of villi and crypts with extension to the muscularis layer. Intestinal damage was mild in group 2 and these histological changes were better than group 1, and worse than group 3. The mean of histologic grade of group 1 was 2.4 (range 2-3), and in the group 2, it was 1.2 (range 0-2). A difference was found when two groups were compared with each other (P < 0.05). In an experimental model of NEC, G-CSF could have a protective effect on intestinal damage.