TUMOR-NECROSIS-FACTOR-ALPHA EXPRESSION IN ISCHEMIC NEURONS
TUMOR-NECROSIS-FACTOR-ALPHA EXPRESSION IN ISCHEMIC NEURONS
复制标题
DOI：
10.1161/01.str.25.7.1481
复制
发表时间：
1994-07-01
期刊：
影响因子：
8.3
通讯作者：
FEUERSTEIN, GZ
中科院分区：
文献类型：
--
作者：
LIU, T;CLARK, RK;FEUERSTEIN, GZ
背景与目的肿瘤坏死因子-α是一种具有多种促炎作用的细胞因子，包括内皮细胞白细胞黏附分子的表达。鉴于白细胞在缺血性脑损伤中的渗入，本研究旨在探讨大鼠局灶性卒中后及白细胞聚集前脑组织中是否存在肿瘤坏死因子-α信使RNA及其多肽的表达。结果大鼠大脑中动脉闭塞后1h即可检测到大鼠巨噬细胞表达的肿瘤坏死因子-α(TNF-α)mRNA的表达，并与正常对照细胞的表达进行了比较。3小时后，缺血区大脑皮层组织中肿瘤坏死因子-α基因表达水平显著升高(29+/-6%，假手术组为2+/-1%)，12h达高峰(104+/-8%；P
Background and Purpose Tumor necrosis factor-alpha (TNF-alpha) is a cytokine with diverse proinflammatory actions, including endothelial leukocyte adhesion molecule expression. Since leukocytes infiltrate into ischemic brain lesions, the present study was conducted to examine whether TNF-alpha messenger RNA (mRNA) and peptide are expressed in the brain after experimental focal stroke and before leukocyte accumulation.Methods TNF-alpha mRNA and protein expression were monitored in the ischemic and nonischemic cerebral cortex of rats after focal ischemia produced by permanent middle cerebral artery occlusion. The effect of TNF-alpha administered by microinjection into the brain cortex on leukocyte adherence to brain capillaries was also studied.Results Induction of TNF-alpha mRNA, normalized to a standard reference rat macrophage TNF-alpha mRNA, was detected as early as 1 hour after middle cerebral artery occlusion. TNF-alpha mRNA was elevated by 3 hours (29+/-6% versus 2+/-1% in sham-operated rats) only in the ischemic cortex, with peak expression at 12 hours (104+/-8%; P