Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.
Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.
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通过诱变和荧光结合分析剖析细胞视黄酸结合蛋白 II 的关键结合决定因素。
DOI：
10.1002/prot.22334
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
Borhan,Babak
中科院分区：
文献类型：
--
作者：
Vasileiou,Chrysoula;Lee,KinSingStephen;Crist,RachaelM;Vaezeslami,Soheila;Goins,SarahM;Geiger,JamesH;Borhan,Babak
对视黄酸与细胞视黄酸结合蛋白 II (CRABPII) 突变体的结合进行评估，以更好地了解直接蛋白质/配体相互作用的重要性。 Arg111 对于蛋白质正确结构和功能的重要作用已得到验证，并且已鉴定出直接影响视黄酸结合的其他残基。此外，通过提供 Glu 残基形式的羧酸二聚体伴侣，可以挽救视黄酸与缺乏所有先前鉴定的相互作用氨基酸的 CRABPII 突变体的结合。蛋白质 2009。© 2008 Wiley-Liss, Inc.
The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue. Proteins 2009. © 2008 Wiley‐Liss, Inc.