PRODUCTION OF OSTEOCALCIN BY HUMAN-BONE CELLS-INVITRO - EFFECTS OF 1,25(OH)2D3, 24,25(OH)2D3, PARATHYROID-HORMONE, AND GLUCOCORTICOIDS
PRODUCTION OF OSTEOCALCIN BY HUMAN-BONE CELLS-INVITRO - EFFECTS OF 1,25(OH)2D3, 24,25(OH)2D3, PARATHYROID-HORMONE, AND GLUCOCORTICOIDS
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DOI：
10.1016/0221-8747(84)90064-x
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
RUSSELL, RGG
中科院分区：
文献类型：
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作者：
BERESFORD, JN;GALLAGHER, JA;RUSSELL, RGG
从人骨培养细胞，其具有成骨细胞的几个特征，包括产生骨钙素（骨Gla蛋白）的能力。在这些培养物中，骨钙素的产生依赖于1，25（OH）2D 3 [1，25-二羟基维生素D3]，但不受24，25（OH）2D 3 [24，25-二羟基维生素D3]单独或与1，25（OH）2D 3组合的影响。两种糖皮质激素泼尼松龙和地夫可特以剂量依赖性方式（10-9-10-6 M）逆转1，25（OH）2D 3对骨钙素合成的刺激。甲状旁腺激素还以剂量依赖性方式（0.2-5 IU/ml）抑制骨钙素的产生。人骨细胞培养物在体外研究骨钙素和其他骨蛋白产生的激素和药理学调节方面可能具有相当大的价值。
Cells were cultured from human bone that possess several characteristics of osteoblasts, including the capacity to produce osteocalcin (bone Gla protein). In these cultures the production of osteocalcin is dependent on 1,25(OH)2D3 [1,25-dihydroxy vitamin D3] but is not affected by 24,25(OH)2D3 [24,25-dihydroxy-vitamin D3] either alone or in combination with 1,25(OH)2D3. Two glucocorticoids, prednisolone and deflazacort, reverse the stimulation of osteocalcin synthesis by 1,25(OH)2D3 in a dose-dependent manner (10-9-10-6 M). Parathyroid hormone also inhibits osteocalcin production in a dose-dependent fashion (0.2-5 IU/ml). Human bone cell cultures may be of considerable value in investigating the hormonal and pharmacologic regulation of the production of osteocalcin and other bone proteins in vitro.