Detection and localisation of HIV-1 DNA and RNA in fixed adult AIDS brain by polymerase chain reaction/in situ hybridisation technique
Detection and localisation of HIV-1 DNA and RNA in fixed adult AIDS brain by polymerase chain reaction/in situ hybridisation technique
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DOI：
10.1007/s004010051113
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发表时间：
1999-11-01
影响因子：
12.7
通讯作者：
Scaravilli, F
中科院分区：
文献类型：
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作者：
An, SF;Groves, M;Scaravilli, F
在艾滋病患者的大脑中，HIV-I以生产性形式存在于单核细胞中。一个仍然需要澄清的问题是，艾滋病毒是否定位于单核细胞以外的细胞中。通过聚合酶链式反应/原位杂交(聚合酶链式反应/原位杂交)的基因扩增可以阐明这一点。在这项研究中，使用了来自10名成年艾滋病患者的福尔马林固定、石蜡包埋的脑组织。其中5例显示有艾滋病毒脑炎(蜂窝)的证据，5例未显示任何异常。套式聚合酶链式反应在所有蜂房病例和3例无蜂窝病例中检测到HIV-1DNA。7例同时检测到HIV-1DNA和RNA(7例均为管内HIV-1DNA阳性)。白质的信号高于灰质。HIV-1DNA存在于小胶质细胞、巨噬细胞、血管周围细胞、多核巨细胞(MGC)和CD68阴性细胞中。部分鉴定为内皮细胞、星形胶质细胞和少突胶质细胞。RT-PCR-IS在巨噬细胞、MGC、内皮细胞和神经胶质细胞中呈阳性反应。这些结果证实了内皮细胞和其他神经胶质细胞的感染，并为病毒进入中枢神经系统的途径和疾病的发病机制提供了线索。这项研究没有提供任何令人信服的证据支持HIV-1感染神经元。
In the brain of patients with AIDS, HIV-I is localised in a productive form in mononuclear cells. One issue that still needs clarification is whether HIV is localised in cells other than those of mononuclear lineage. Gene amplification by polymerase chain reaction/in situ hybridisation (PCR-IS) could shed light on it. In this study, formalin-fixed, paraffin-embedded brain tissue from ten adult AIDS sufferers was used. Five of them showed evidence of HIV encephalitis (HIVE), five did not show any abnormality. Nested PCR revealed HIV-1 DNA in all HIVE cases and in three of the group without HIVE. HIV-1 DNA and RNA were also detected in situ in seven cases (all seven were also HIV-1 DNA positive in tube). A higher signal was located in the white than in the grey matter. HIV-1 DNA was found in microglia, macrophages, perivascular cells, multinucleated gaint cells (MGC) and in CD68-negative cells. Some of them were identified as endothelial cells, astrocytes and oligodendrocytes. Reverse transcriptase-PCR-IS was positive in macrophages, MGC, endothelial and glial cells. These results confirm infection of endothelial cells and other glial cells and give clues about the route of entry of virus into the central nervous system and the pathogenesis of the disease. This study did not give any convincing evidence supporting an infection of neurons by HIV-1.