Evaluation of adjuvants for protein vaccines against tuberculosis in guinea pigs
Evaluation of adjuvants for protein vaccines against tuberculosis in guinea pigs
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DOI：
10.1016/s0264-410x(02)00548-0
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发表时间：
2003-02-14
期刊：
影响因子：
5.5
通讯作者：
Chambers, MA
中科院分区：
文献类型：
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作者：
Hogarth, PJ;Jahans, KJ;Chambers, MA
针对结核病的亚单位疫苗显示出前景，但需要与佐剂一起施用以刺激相关的免疫应答以提供保护。豚鼠是评价用强毒分枝杆菌产气攻击的保护性免疫的首选模型，但很少有研究来确定该物种中疫苗筛选的合适佐剂。在这里，我们比较了几种佐剂诱导T细胞对豚鼠培养滤液蛋白的反应的功效。我们报告说，测试的几种佐剂，最有前途的是CpG ODN配制在水乳液。这种佐剂诱导的I型T细胞反应相当于FIA的反应，如通过迟发型超敏反应（DTH）、抗原特异性T细胞增殖和抗原特异性IgG1和IgG2反应所测量的。这些数据证明了基于CpG基序的佐剂用于在豚鼠中进行TB疫苗筛选以及需要1型T细胞应答的其他疾病的潜力。皇冠版权所有（C）2002由爱思唯尔科学有限公司出版。保留所有权利。
Subunit vaccines against tuberculosis show promise but require administration with adjuvants to stimulate relevant immune responses for protection. Guinea pigs are the model of choice for evaluating protective immunity to aerogenic challenge with virulent mycobacteria, but few studies have been undertaken to identify suitable adjuvants for vaccine screening in this species. Here, we compare the efficacy of several adjuvants to induce T cell responses to culture filtrate protein in guinea pigs. We report that of several adjuvants tested, the most promising was CpG ODN formulated in an aqueous emulsion. This adjuvant induced type I T cell responses equivalent to that of FIA, as measured by delayed-type hypersensitivity reactions (DTH), antigen-specific T cell proliferation and antigen-specific IgG1 and IgG2 responses. These data demonstrate the potential for CpG motif based adjuvants for use in TB vaccine screening in guinea pigs, and other diseases where a type 1 T cell response is required. Crown Copyright (C) 2002 Published by Elsevier Science Ltd. All rights reserved.