Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome.
Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome.
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苜蓿银纹夜蛾核型多角体病毒基因组 HindIII F 区的核苷酸序列和转录图谱。
DOI：
10.1099/0022-1317-72-2-285
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Weaver,RF
中科院分区：
文献类型：
--
作者：
Tilakaratne,N;Hardin,SE;Weaver,RF
采用DNA测序、Northern blot分析、S1定位和引物延伸分析相结合的方法，研究了加利福尼亚核多角体病毒基因组hindiii F区2.95 kbEcoRI-SalI片段（0.7 ~ 3.0个图谱单位）的基因组织。除了先前报道的两个不同的重叠转录本[向左早（ES1）和向右晚（ES2）]外，第三个转录本存在于18 h p.i.，也向左运行，在3 '端与ES1重叠1600个核苷酸（nt），被映射到该区域。DNA序列显示存在三个长度显著的开放阅读框（orf）。ORF-1和ORF-2对应左侧转录本，分别编码151个和329个氨基酸的潜在多肽。编码167个氨基酸的潜在多肽的ORF-3位于另一条链上的一个没有转录物定位数据的区域。然而，保守的晚期基因启动子/帽位点序列（ATAAG）存在于ORF-3起始点上游23nt处。
The organization of genes in the 2.95 kbEcoRI-SalI fragment (0.7 to 3.0 map units) located within theHindIII F region of theAutographa californicanuclear polyhedrosis virus genome was studied by a combination of DNA sequencing, Northern blot analysis, S1 mapping and primer extension analysis. In addition to the two divergent overlapping transcripts [leftward early (ES1) and rightward late (ES2)] previously reported, a third transcript which is present at 18 h p.i. and which also runs leftward, overlapping ES1 by 1600 nucleotides (nt) at the 3′ end, was mapped to this region. The DNA sequence revealed the presence of three open reading frames (ORFs) of significant length. ORF-1 and ORF-2 correspond to the leftward transcripts, and code for potential polypeptides of 151 and 329 amino acids, respectively. ORF-3 which codes for a potential polypeptide of 167 amino acids is located on the opposite strand in a region for which no transcript mapping data are available. However, the conserved late gene promoter/cap site sequence (ATAAG) is present 23 nt upstream of the start of ORF-3.