Increased thalamic inhibition in the absence seizure prone DBA/2J mouse.
Increased thalamic inhibition in the absence seizure prone DBA/2J mouse.
复制标题
缺乏癫痫发作倾向的 DBA/2J 小鼠的丘脑抑制增加。
DOI：
10.1111/j.1528-1167.2008.01536.x
复制
发表时间：
2008
期刊：
影响因子：
5.6
通讯作者：
Petrou,Steven
中科院分区：
文献类型：
--
作者：
Tan,HeneuO;Reid,ChristopherA;Chiu,Cindy;Jones,MathewV;Petrou,Steven
遗传背景在患者和动物模型中产生不同的癫痫结果中起着重要作用。这种异质性背后的神经生物学机制尚不清楚。在这里，我们比较了两种常用近交系小鼠品系（C57B/6 和倾向于棘波放电 (SWD) 的 DBA/2J）丘脑皮质回路内的 GABA 突触特性。菌株之间的差异以区域特异性方式出现在微型抑制性突触后电流（mIPSC）的幅度、动力学和频率上。突触抑制的最大差异出现在丘脑中，与 C57B/6 相比，DBA/2J 小鼠的 mIPSC 频率增加了一倍。脑电图分析显示，DBA/2J 小鼠在非快速动眼睡眠期间，6-12 Hz 频段的功率较高。 DBA2/J 应变对形成 SWD 的敏感性增加以及 6-12 Hz EEG 功率的增加可能是由于 GABA 能输入增加导致腹基底丘脑超同步所致。
Genetic background plays a significant role in producing variable seizure outcomes in patients and animal models. The neurobiological mechanism underlying this heterogeneity is not clear. Here we compare GABAergic synaptic properties within the thalamocortical circuit of two commonly used inbred mice strains, the C57B/6 and spike‐and‐wave discharge (SWD) prone DBA/2J. Differences between the strains occur in amplitude, kinetics, and frequency of miniature inhibitory postsynaptic currents (mIPSCs) in a region‐specific manner. The biggest difference in synaptic inhibition was seen in the thalamus where DBA/2J mice showed a doubling of mIPSC frequency when compared to C57B/6. EEG analysis revealed higher power in the 6–12 Hz band during non–rapid eye movement sleep in DBA/2J mice. Increased susceptibility of the DBA2/J strain to develop SWDs and increase in the 6–12 Hz EEG power may be due to a hypersynchronous ventrobasal thalamus as a consequence of increased GABAergic input.