Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission
Isolation of Leptospira interrogans Hardjoprajitno from vaginal fluid of a clinically healthy ewe suggests potential for venereal transmission
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DOI：
10.1099/jmm.0.065466-0
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发表时间：
2014-09-01
影响因子：
3
通讯作者：
Lilenbaum, W.
中科院分区：
文献类型：
--
作者：
Director, A.;Penna, B.;Lilenbaum, W.
对同一群15只已知钩端螺旋体病血清反应的成年母羊进行了研究。收集每只动物的尿液和阴道液，使用细菌培养和传统PCR方法检测钩端螺旋体的存在。从一只未怀孕母羊的阴道液样本中获得一株钩端螺旋体纯培养物。经rrs和secY基因的DNA测序、可变数目串联重复序列（VNTR）分析和血清分型鉴定，该分离株为疑问钩端螺旋体Sejroe血清型Hardjo型Hardjoprajitno。本报告表明，在一只母羊的阴道液中存在活的钩端螺旋体，这表明在绵羊中存在钩端螺旋体性病传播的可能性。
A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of Leptospira sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the rrs and secY genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as Leptospira interrogans serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable Leptospira in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.