The male-produced aggregation pheromone of the bean flower thrips Megalurothrips usitatus in China: identification and attraction of conspecifics in the laboratory and field
The male-produced aggregation pheromone of the bean flower thrips Megalurothrips usitatus in China: identification and attraction of conspecifics in the laboratory and field
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DOI：
10.1002/ps.5844
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发表时间：
2020-04-21
影响因子：
4.1
通讯作者：
Shi, Wangpeng
中科院分区：
文献类型：
--
作者：
Liu, Pingping;Qin, Zifang;Shi, Wangpeng
背景：大尿蓟马（meggalurothrips usitatus）通常表现出聚集行为，这可能是由雄性产生的聚集信息素介导的。聚集信息素具有物种特异性，可用于开发商业诱饵，用于监测和大规模捕获害虫。鉴定了四种蓟马聚集信息素的有效成分。然而，目前尚不清楚黑穗病菌产生的聚集信息素的成分。结果y管嗅探结果显示，雄性和未交配的雌性褐家蝇对雄性挥发物有明显的吸引作用，而对雌性挥发物没有明显的吸引作用。触角电图（EAG）分析也支持了这一点。气相色谱-天线电图耦合检测（GC-EAD）表明，男性特有气味的一种成分引起了显著的电生理反应。该化合物被鉴定为(2E,6E)-法尼酯乙酸酯，其结构与其他报道的蓟马聚集信息素的活性成分不同。随着合成(2E,6E)-法尼酯乙酸酯剂量的增加，家蚕的触角电反应增强。此外，该化合物在实验室行为生物测定中显著吸引成人。在田间条件下，合成(2E,6E)-法尼酯乙酸酯粘捕器捕获的褐僵菌数量是对照的1.5 ~ 7倍，且该化合物60 μ g的效果至少持续6天。结论(2E,6E)-法尼酯醋酸酯被鉴定为雄蜂产生的聚集信息素。在实验室和野外条件下，该方法均可吸引该蓟马物种，表明其在控制马尾蓟马种群方面具有相当大的商业应用潜力。
BACKGROUND Thrips, Megalurothrips usitatus, usually display aggregation behavior, which is probably mediated by a male-produced aggregation pheromone. Aggregation pheromones are species-specific, and can be used to develop commercial lures for monitoring and mass-trapping of pests. The active components of the aggregation pheromone for four thrips species have been identified. However, the components of M. usitatus-produced aggregation pheromone are still not clear.RESULT Y-tube olfactometer assays showed that both male and virgin female M. usitatus were significantly attracted to male but not female volatiles. This was additionally supported by electroantennogram (EAG) assays. Coupled gas chromatography-electroantennogram detection (GC-EAD) showed that one component of male-specific odors elicited a significant electrophysiological response. This compound was characterized as (2E,6E)-farnesyl acetate, which is structurally different from the active components of the aggregation pheromones of other reported thrips species. Electroantennal responses of M. usitatus increased with increasing doses of synthetic (2E,6E)-farnesyl acetate. Additionally, this compound significantly attracted adults in laboratory behavioral bioassays. Under field conditions, sticky traps with synthetic (2E,6E)-farnesyl acetate caught 1.5-7-fold more M. usitatus than controls, and this effect of the compound at a dose of 60 mu g lasted at least 6 days.CONCLUSION (2E,6E)-Farnesyl acetate was identified as the male-produced aggregation pheromone of M. usitatus. It could attract this thrips species under laboratory and field conditions, suggesting considerable potential as a commercial application to control M. usitatus populations.