Rice microRNA osa-miR1848 targets the obtusifoliol 14α-demethylase gene OsCYP51G3 and mediates the biosynthesis of phytosterols and brassinosteroids during development and in response to stress
Rice microRNA osa-miR1848 targets the obtusifoliol 14α-demethylase gene OsCYP51G3 and mediates the biosynthesis of phytosterols and brassinosteroids during development and in response to stress
复制标题
水稻 microRNA osa-miR1848 靶向钝叶醇 14α-脱甲基酶基因 OsCYP51G3，并在发育过程中和应激反应中介导植物甾醇和油菜素类固醇的生物合成。
DOI：
10.1111/nph.13513
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发表时间：
2015-11-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Mingyong
中科院分区：
文献类型：
--
作者：
Xia, Kuaifei;Ou, Xiaojing;Zhang, Mingyong
植物甾醇是油菜素类固醇 (BR) 生物合成的膜成分或前体。由于它们无法长距离运输，因此它们的体内平衡通过其生物合成和新陈代谢受到严格控制。然而，尚不清楚 microRNA 是否参与其稳态调节。用 microRNA osa-miR1848 及其靶标 obtusifoliol 14 α-去甲基酶基因 OsCYP51G3 转化的水稻 (Oryza sativa) 植物被用来研究 osa-miR1848 在植物甾醇生物合成调节中的作用。osa-miR1848 指导 OsCYP51G3 mRNA裂解调节水稻植物甾醇和 BR 生物合成。 OsCYP51G3 作为 osa-miR1848 靶标之一的作用得到了转基因水稻植物中 osa-miR1848 和 OsCYP51G3 相反的表达模式以及 OsCYP51G3 mRNA 切割位点的鉴定的支持。 osa-miR1848增加和OsCYP51G3表达减少会降低植物甾醇和BR浓度，并引起与植物甾醇和BR缺乏相关的典型表型变化，包括矮化植物、直立叶片、半不育花粉粒和较短细胞。 osa-miR1848的昼夜节律表达调节发育器官中OsCYP51G3转录本的昼夜丰度，以及OsCYP51G3对盐胁迫的反应。我们认为osa-miR1848在转录后调节OsCYP51G3表达，并介导植物甾醇和BR生物合成。 osa-miR1848 和 OsCYP51G3 可能在水稻育种中具有潜在的应用，以调节叶角、种子的大小和质量。
Phytosterols are membrane components or precursors for brassinosteroid (BR) biosynthesis. As they cannot be transported long distances, their homeostasis is tightly controlled through their biosynthesis and metabolism. However, it is unknown whether microRNAs are involved in their homeostatic regulation.Rice (Oryza sativa) plants transformed with microRNA osa-miR1848 and its target, the obtusifoliol 14 alpha-demethylase gene, OsCYP51G3, were used to investigate the role of osa-miR1848 in the regulation of phytosterol biosynthesis.osa-miR1848 directs OsCYP51G3 mRNA cleavage to regulate phytosterol and BR biosynthesis in rice. The role of OsCYP51G3 as one of the osa-miR1848 targets is supported by the opposite expression patterns of osa-miR1848 and OsCYP51G3 in transgenic rice plants, and by the identification of OsCYP51G3 mRNA cleavage sites. Increased osa-miR1848 and decreased OsCYP51G3 expression reduced phytosterol and BR concentrations, and caused typical phenotypic changes related to phytosterol and BR deficiency, including dwarf plants, erect leaves, semi-sterile pollen grains, and shorter cells. Circadian expression of osa-miR1848 regulated the diurnal abundance of OsCYP51G3 transcript in developing organs, and the response of OsCYP51G3 to salt stress.We propose that osa-miR1848 regulates OsCYP51G3 expression posttranscriptionally, and mediates phytosterol and BR biosynthesis. osa-miR1848 and OsCYP51G3 might have potential applications in rice breeding to modulate leaf angle, and the size and quality of seeds.