The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex.
The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex.
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DOI：
10.1083/jcb.108.4.1271
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发表时间：
1989-04
期刊：
影响因子：
--
通讯作者：
Greene R
中科院分区：
文献类型：
--
作者：
Bankaitis VA;Malehorn DE;Emr SD;Greene R
我们获得并鉴定了SEC14的一个基因组克隆，SEC14是酿酒酵母（Saccharomyces cerevisiae）的一个基因，其产物是酵母分泌蛋白从高尔基体输出所必需的。基因破坏实验表明，SEC14是酵母营养生长的一个必需基因。核苷酸序列分析显示，SEC14结构基因内存在一个内含子，并预测会合成一种分子量为35kD的亲水性多肽。经免疫沉淀实验证实，SEC14p是一种未糖基化的多肽，表观分子量约为37kD，在亚细胞分级分离实验中主要表现为一种胞质蛋白。这些数据与SEC14p是一种促进酵母高尔基体蛋白质输出的胞质因子这一观点相符。另外的放射性标记实验还显示，在从乳酸克鲁维酵母（Kluyveromyces lactis）和粟酒裂殖酵母（Schizosaccharomyces pombe）制备的提取物中存在与SEC14p相关的多肽。此外，乳酸克鲁维酵母的SEC14p能够功能性互补酿酒酵母sec14ts的缺陷。这些数据表明，在这些酵母物种中，SEC14p的结构和功能具有一定程度的保守性。
We have obtained and characterized a genomic clone of SEC14, a Saccharomyces cerevisiae gene whose product is required for export of yeast secretory proteins from the Golgi complex. Gene disruption experiments indicated that SEC14 is an essential gene for yeast vegetative growth. Nucleotide sequence analysis revealed the presence of an intron within the SEC14 structural gene, and predicted the synthesis of a hydrophilic polypeptide of 35 kD in molecular mass. In confirmation, immunoprecipitation experiments demonstrated SEC14p to be an unglycosylated polypeptide, with an apparent molecular mass of some 37 kD, that behaved predominantly as a cytosolic protein in subcellular fractionation experiments. These data were consistent with the notion that SEC14p is a cytosolic factor that promotes protein export from yeast Golgi. Additional radiolabeling experiments also revealed the presence of SEC14p-related polypeptides in extracts prepared from the yeasts Kluyveromyces lactis and Schizosaccharomyces pombe. Furthermore, the K. lactis SEC14p was able to functionally complement S. cerevisiae sec14ts defects. These data suggested a degree of conservation of SEC14p structure and function in these yeasts species.