Production of bioactive peptide hydrolysates from deer, sheep, pig and cattle red blood cell fractions using plant and fungal protease preparations
Production of bioactive peptide hydrolysates from deer, sheep, pig and cattle red blood cell fractions using plant and fungal protease preparations
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DOI：
10.1016/j.foodchem.2016.02.020
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发表时间：
2016-07-01
期刊：
影响因子：
8.8
通讯作者：
Bekhit, Alaa El-Din A.
中科院分区：
文献类型：
--
作者：
Bah, Clara S. F.;Carne, Alan;Bekhit, Alaa El-Din A.
采用植物源（木瓜蛋白酶和菠萝蛋白酶）和真菌源（FP 400和FPII）的蛋白酶制剂水解鹿、羊、猪和牛屠宰场来源的血液中分离的红细胞组分（RBCF）。在水解1、2、4和24 h后，对所得肽水解物的抗氧化活性和抑菌活性进行了研究。三氯乙酸可溶性肽在水解期间的增加使用邻苯二甲醛（OPA）测定进行了检查，并使用SDS-PAGE说明了水解曲线。木瓜蛋白酶产生的RBCF水解产物表现出更高的铁还原抗氧化能力（FRAP）和氧自由基吸收能力（ORAC）相比，菠萝蛋白酶，FP 400和FPII产生的。在一定浓度下，FP 400和FPII酶解RBCF 24 h后，对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌均有抑制作用。结果表明，利用植物或真菌来源的蛋白酶可以产生具有抗氧化和抗菌活性的动物血液水解物。(C)2016爱思唯尔有限公司版权所有
Protease preparations from plant (papain and bromelain) and fungal (FP400 and FPII) sources were used to hydrolyze the red blood cell fractions (RBCFs) separated from deer, sheep, pig, and cattle abattoir-sourced blood. After 1, 2, 4 and 24 h of hydrolysis, the antioxidant and antibacterial activities of the peptide hydrolysates obtained were investigated. The increase in trichloroacetic acid-soluble peptides over the hydrolysis period was examined using the o-phthaldialdehyde (OPA) assay and the hydrolysis profiles were illustrated using SDS-PAGE. Papain generated RBCF hydrolysates exhibited higher ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) compared to those generated with bromelain, FP400 and FPII. At certain concentrations, 24 h hydrolysates of RBCF using FP400 and FPII were able to inhibit the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results indicated that the use of proteases from plant or fungal sources can produce animal blood hydrolysates with antioxidant and antimicrobial activities. (C) 2016 Elsevier Ltd. All rights reserved.