YAC transgenic analysis reveals Wilms' Tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb
YAC transgenic analysis reveals Wilms' Tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb
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DOI：
10.1016/s0925-4773(98)00188-9
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发表时间：
1998-12-01
影响因子：
2.6
通讯作者：
Hastie, ND
中科院分区：
文献类型：
--
作者：
Moore, AW;Schedl, A;Hastie, ND
Wilms' Tumour 1基因（WT 1）是泌尿生殖系统器官发育所必需的基因。为了研究其调节和表达，我们创建了几个转基因小鼠品系，其中含有由人WT 1启动子驱动的β-半乳糖苷酶报告基因。一个5 kb的启动子弱重演的内源性Wt 1表达模式的一个子集。相比之下，470和280 kb的YAC转基因复制了正确的模式，具有高活性，并突出了新的表达位点。Wt 1在横隔中表达，揭示了其突变如何导致骨缺损，Wt 1在肢体中的表达在软骨形成和凋亡区域之间划定了一个区域。最后，Wt 1在体腔上皮来源的间充质细胞中表达。基于这些和进一步的数据，我们讨论了Wt 1的作用，上皮TT间质转化。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Wilms' Tumour 1 gene (WT1) is required for the con ect development of the urogenital system. To examine its regulation and expression, we created several transgenic mouse lines containing a P-galactosidase reporter driven by the human WT1 promoter. A 5 kb promoter weakly recapitulated a subset of the endogenous Wt1 expression pattern. In contrast, 470 and 280 kb YAC transgenes reproduced the correct pattern with high activity and highlighted new expression sites. Wt1 is expressed in the septum transversum revealing how its mutation causes diaphragmatic defects, Wt1 expression in the limb demarcates a zone between chondrogenic and apoptotic domains. Finally, Wt1 is expressed in mesenchymal cells derived from the coelomic epithelium. Based upon these and further data we discuss a Wt1 role in epithelial tt mesenchymal transitions. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.