Inhibition by cyclosporin A and butylated hydroxytoluene of the inner mitochondrial membrane permeability transition induced by adriamycin aglycones.

Inhibition by cyclosporin A and butylated hydroxytoluene of the inner mitochondrial membrane permeability transition induced by adriamycin aglycones.
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环孢菌素 A 和丁基羟基甲苯对阿霉素苷元诱导的线粒体内膜通透性转变的抑制作用。

DOI:
10.1016/0006-2952(90)90595-c
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发表时间:
1990
影响因子:
5.8
通讯作者:
Sokolove,PM
Sokolove,PM
中科院分区:
医学2区
文献类型:
--
作者:
Sokolove,PM

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MethodsMitochondria were isolated from the hearts of male Sprague-Dawley rats by a procedure[lo] which yields a mixed population of interfibrillar and subsarcolemmal organelles. Liver mitochondria were isolated according to Johnson and Lardy [18], but using the same buffers as for the heart preparation. Experimentswith heart mitochondria were carried out in 2.5 mL of a standard resin (Chelex-100) treated buffer, which consisted of 100mM sucrose, 50mM KCI, 20mM MOPS-KOH(pH 7.2). and 1.7 mM KH, PO,, to which was added 0.8, uM rotenone and mitochondrial protein equivalent to 0.2 mg/mL. For
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