Hyperplasia precedes increased glomerular filtration rate in rat remnant kidney.
Hyperplasia precedes increased glomerular filtration rate in rat remnant kidney.
复制标题
在大鼠残肾中，增生先于肾小球滤过率增加。
DOI：
10.1038/ki.1990.43
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发表时间：
1990
影响因子：
19.6
通讯作者：
Simpson,DP
中科院分区：
文献类型：
--
作者：
Miskell,CA;Simpson,DP
大鼠残肾增生先于肾小球滤过率增加。用~ 3 H-胸苷（~ 3 H-T）检测了单侧肾切除（UNI）、六分之五肾切除（R）和假手术（S）大鼠的DNA合成。24、48或72小时后，动物被注入14 C-菊糖、PAH和3 H-T，并获得清除率。处死前，注射印度墨水进行肾小球计数。到24小时，UNI患者的肾小球滤过率/肾单位显著增加。然而，在R中，每个肾单位的肾小球滤过率显著低于S，直到72小时。UNI中每个肾单位的总μg DNA无变化，但在R中与S相比在所有时间均显著增加。UNI中DNA中的3 H-T掺入量是S的两倍，在24小时时R中比S高5倍以上;在48和72小时时，R中这种显著增加持续存在。放射自显影证实DNA主要由肾小管细胞而不是浸润细胞合成。这些结果表明，在代偿性肾生长的增生有关的组织去除的数量，并在残肾，DNA合成之前的肾小球滤过率每肾单位的代偿性增加。
Hyperplasia precedes increased glomerular filtration rate in rat remnant kidney. Using3H-thymidine (3H-T), we examined DNA synthesis in rats subjected to either uninephrectomy (UNI), five-sixths nephrectomy (R) or sham (S) surgery. Twenty-four, 48, or 72 hours later, animals were infused with14C-inulin, PAH and3H-T and clearances obtained. Prior to sacrifice, India ink was injected for glomerular counting. By 24 hours, glomerular filtration rate per nephron was significantly increased in UNI. However, in R, glomerular filtration rate per nephron was significantly lower than S until 72 hours. Total µg DNA per nephron was unchanged in UNI but significantly increased in R compared to S at all times.3H-T incorporation into DNA was twice as great in UNI as in S and was over five-fold greater at 24 hours in R than in S; this marked increase persisted in R at 48 and 72 hours. Autoradiographs confirmed that DNA was synthesized predominantly by renal tubular cells and not infiltrating cells. These results indicate that hyperplasia in compensatory renal growth is related to the quantity of tissue removed and that, in the remnant kidney, DNA synthesis precedes the compensatory increase in glomerular filtration rate per nephron.