Chromatin structure of altered yeast centromeres.
Chromatin structure of altered yeast centromeres.
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改变的酵母着丝粒的染色质结构。
DOI：
10.1073/pnas.85.1.175
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发表时间：
1988
影响因子：
11.1
通讯作者：
Bloom,K
中科院分区：
文献类型：
--
作者：
Saunders,M;Fitzgerald-Hayes,M;Bloom,K
我们研究了染色质结构的野生型和突变改变的着丝粒序列在酵母酿酒酵母通过使用间接末端标记映射策略。来自染色体III（CEN 3）的野生型着丝粒DNA表现出220-250个碱基对长的抗核酸酶染色质结构，其以保守的着丝粒DNA元件（CDE）III为中心。CDE III中的一个点突变将中心胞苷改变为胸苷，并完全破坏了着丝粒功能，该点突变已经失去了通常与野生型着丝粒相关的染色质构象。第二个保守的DNA元件，CDE I，在空间上与CDE III分开78-86个富含A + T的碱基对，称为CDE II。CDE II的序列和空间要求不太严格; CDE II长度和序列的改变可以在有限程度上耐受。在两个CDE II CEN 3突变中，核酸酶抗性核心的尺寸发生改变。保留部分着丝粒功能的两个CDE I缺失突变也显示出大小和强度降低的核酸酶抗性区域。来自许多这样的改变的着丝粒的结果表明受保护的核心的存在和着丝粒功能之间的相关性。
We have investigated the chromatin structure of wild-type and mutationally altered centromere sequences in the yeast Saccharomyces cerevisiae by using an indirect end-labeling mapping strategy. Wild-type centromere DNA from chromosome III (CEN3) exhibits a nuclease-resistant chromatin structure 220-250 base pairs long, centered around the conserved centromere DNA element (CDE) III. A point mutation in CDE III that changes a central cytidine to a thymidine and completely disrupts centromere function has lost the chromatin conformation typically associated with the wild-type centromere. A second conserved DNA element, CDE I, is spatially separated from CDE III by 78-86 A + T-rich base pairs, which is termed CDE II. The sequence and spatial requirements for CDE II are less stringent; alterations in CDE II length and sequence can be tolerated to a limited extent. Nuclease-resistant cores are altered in dimension in two CDE II CEN3 mutations. Two CDE I deletion mutations that retain partial centromere function also show nuclease-resistant regions of reduced size and intensity. The results from a number of such altered centromeres indicate a correlation between the presence of a protected core and centromere function.