Formation of giant polymer vesicles by simple double emulsification using block copolymers as the sole surfactant†
Formation of giant polymer vesicles by simple double emulsification using block copolymers as the sole surfactant†
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DOI：
10.1039/d1sm00301a
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发表时间：
2021-03-23
期刊：
影响因子：
3.4
通讯作者：
Landfester, Katharina
中科院分区：
文献类型：
--
作者：
Houbrechts, Mazarine;Caire da Silva, Lucas;Landfester, Katharina
模拟细胞膜功能的聚合物囊泡可以通过两亲性嵌段共聚物的自组装而获得。聚合物囊泡的核壳结构、半透过性和表面化学可调等类细胞特性使其成为人工细胞的极佳构建块。然而，标准的聚合物囊泡制备方法可能会耗时、需要特殊设备，或者对纳米材料和蛋白质等大组分的包封率较低。在这里，我们介绍了一种基于简单的双重乳化(SDE)方法的新的包覆策略，该方法允许在基本的实验室设备下在短时间内形成巨大的聚合物囊泡。SDE方法需要单一的低分子量嵌段共聚物，它具有大分子表面活性剂和膜构建块的双重作用。产生了直径在20-50微米之间的巨型聚合物囊泡，这使得蛋白质和纳米颗粒可以被包裹起来。为了演示它的实际应用，我们使用SDE方法组装了一个简单的人造细胞，它模仿了一个两步酶促级联反应。本文介绍的SDE方法介绍了一种简单、快速制造合成室的新工具。
Polymer vesicles that mimic the function of cell membranes can be obtained through the self-assembly of amphiphilic block copolymers. The cell-like characteristics of polymer vesicles, such as the core-shell structure, semi-permeability and tunable surface chemistry make them excellent building blocks for artificial cells. However, the standard preparation methods for polymer vesicles can be time consuming, require special equipment, or have low encapsulation efficiency for large components, such as nanomaterials and proteins. Here, we introduce a new encapsulation strategy based on a simple double emulsification (SDE) approach which allows giant polymer vesicles to be formed in a short time and with basic laboratory equipment. The SDE method requires a single low molecular weight block copolymer that has the dual role of macromolecular surfactant and membrane building block. Giant polymer vesicles with diameters between 20-50 mu m were produced, which allowed proteins and nanoparticles to be encapsulated. To demonstrate its practical application, we used the SDE method to assemble a simple artificial cell that mimics a two-step enzymatic cascade reaction. The SDE method described here introduces a new tool for simple and rapid fabrication of synthetic compartments.