Inhibiting complement activation on cells at the step of C3 cleavage
Inhibiting complement activation on cells at the step of C3 cleavage
复制标题
DOI：
10.1016/j.vaccine.2008.11.001
复制
发表时间：
2008-12-30
期刊：
影响因子：
5.5
通讯作者：
Atkinson, John P.
中科院分区：
文献类型：
--
作者：
Liszewski, M. Kathryn;Fang, Celia J.;Atkinson, John P.
补体系统中近一半的蛋白质起着调节作用。 Control at the central step of C3 activation is provided by ail orchestrated interplay of membrane and plasma regulators.采用转染人类调节剂的中国仓鼠卵巢（CHO）细胞的模型系统被用来协助进行功能比较。此外，在该实验设置中，可以改变补体激活的途径和程度，同时监测 C4b/C3b 沉积和裂解以及细胞毒性。这篇综述描述了经验教训以及该模型在功能表征与非典型溶血性尿毒症综合征相关的调节因子突变方面的应用。 (C) 2008 Elsevier Ltd. 保留所有权利。
Nearly half of the proteins in the complement system serve in regulation. Control at the central step of C3 activation is provided by ail orchestrated interplay of membrane and plasma regulators. A model system employing Chinese hamster ovary (CHO) cells transfected with human regulators was employed to assist in making functional comparisons. Also, in this experimental setup, the pathway and magnitude of complement activation can be varied while monitoring C4b/C3b deposition and cleavage as well as cytotoxicity. This review describes lessons learned and the application of this model for functionally characterizing mutations in regulators associated with atypical hemolytic Uremic syndrome. (C) 2008 Elsevier Ltd. All rights reserved.