CATABOLISM OF INDOLE-3-ACETIC-ACID AND 4-CHLOROINDOLE-3-ACETIC AND 5-CHLOROINDOLE-3-ACETIC ACID IN BRADYRHIZOBIUM-JAPONICUM
CATABOLISM OF INDOLE-3-ACETIC-ACID AND 4-CHLOROINDOLE-3-ACETIC AND 5-CHLOROINDOLE-3-ACETIC ACID IN BRADYRHIZOBIUM-JAPONICUM
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DOI：
10.1128/jb.177.20.5762-5766.1995
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发表时间：
1995-10-01
影响因子：
3.2
通讯作者：
JOCHIMSEN, BU
中科院分区：
文献类型：
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作者：
JENSEN, JB;EGSGAARD, H;JOCHIMSEN, BU
大豆慢生根瘤菌（Bradyrhizobium japonicum）的部分菌株具有分解代谢吲哚乙酸（Indoleacetic acid，IAA）、4-氯吲哚乙酸（4-chloro-IAA）和吲哚乙酸（Indoleacetic acid，IAA）的能力利用高效液相色谱和常规质谱（MS-质谱、紫外光谱、质谱、质谱）等方法对代谢产物进行了分离和分析，鉴定的产物表明IAA通过二氧吲哚3-乙酸、二氧吲哚、靛红和2-氨基苯乙醛酸代谢的新的代谢途径（靛红酸）转化为邻氨基苯甲酸，邻氨基苯甲酸被进一步代谢，4-Cl-IAA的降解明显地在4-Cl-二氧吲哚步骤停止，而5-Cl-IAA被代谢成5-Cl-邻氨基苯甲酸。
Some strains of Bradyrhizobium japonicum have the ability to catabolize indole-3-acetic acid, Indoleacetic acid (IAA), 4-chloro-IAA (4-Cl-IAA), and 5-Cl-IAA were metabolized to different extents by strains 61A24 and 110, Metabolites were isolated and analyzed by high-performance liquid chromatography and conventional mass spectrometry (MS) methods, including MS-mass spectroscopy, UV spectroscopy, and high-performance liquid chromatography-MS. The identified products indicate a novel metabolic pathway in which IAA is metabolized via dioxindole 3-acetic acid, dioxindole, isatin, and 2-aminophenyl glyoxylic acid (isatinic acid) to anthranilic acid, which is further metabolized, Degradation of 4-Cl-IAA apparently stops at the 4-Cl-dioxindole step in contrast to 5-Cl-IAA which is metabolized to 5-Cl-anthranilic acid.