Blood removal and water behavior in horse mackerel Trachurus japonicus muscle using water perfusion
Blood removal and water behavior in horse mackerel Trachurus japonicus muscle using water perfusion
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DOI：
10.1016/j.aquaculture.2022.739098
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发表时间：
2022-11
期刊：
影响因子：
4.5
通讯作者：
Yuta Narisawa;Yuki Tsutsumi;Yusa Nakamura;Kigen Takahashi
中科院分区：
文献类型：
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作者：
Yuta Narisawa;Yuki Tsutsumi;Yusa Nakamura;Kigen Takahashi
除血在渔业和水产养殖业中得到了广泛的应用，因为它在储存过程中保持了鱼肌肉的质量，提高了市场价值和海洋资源的有效利用。水灌注，如asTsumoto-shiki，最近作为一种新的血液清除方法引起了人们的关注；然而，到目前为止，其具体效果尚未得到澄清。为了利用水灌注作为一种新的血液去除技术，我们研究了根据tsumoto -shiki修改后的水灌注4-60 s对马鲛鱼日本粗肌血液去除和水行为的影响，并与常规切鳃出血样品进行了比较。使用Tukey-Kramer方法对结果进行了多次比较测试。灌注标本中血红蛋白含量与灌注时间无关，显著低于未抽血对照标本和出血标本（p< 0.05）。灌注样品中的水分含量和可表达滴度随灌注时间的增加而增加。低场核磁共振（LF-NMR）结果显示，灌注30 ~ 60 s后，1ht2弛豫时间延长（p< 0.05）。在磁共振成像（MRI）获得的1ht2加权图像中，灌注30-60 s的样品，在深色肌肉和节段性动静脉中主要观察到高信号强度，表明存在血管渗漏的游离水。因此，与常规出血相比，水灌注是一种有效的血液清除方法。此外，在本研究中，4-10 s的水灌注既可以去除马鲛鱼肌肉中的血液，又可以防止马鲛鱼肌肉中水分含量和可表达滴度增加导致的质量恶化。
Blood removal is widely applied in the fisheries and aquaculture industries because it maintains the quality of fish muscle during storage, which improves the market value and effective utilization of marine resources. Water perfusion, such asTsumoto-shiki, has recently attracted attention as a novel blood removal procedure; however, its detailed effect has not been clarified thus far. Herein, to utilize water perfusion as a novel blood removal technology, the influence of water perfusion according toTsumoto-shikiwith modifications, for 4–60 s on the blood removal and water behavior in horse mackerelTrachurus japonicusmuscle was investigated compared with that of a sample conventionally bled by cutting the gill. The results were subjected to multiple comparison tests using the Tukey–Kramer method. The hemoprotein content in the perfused sample was significantly lower than that in the control sample without blood removal and the bled sample (p< 0.05), independent of the perfusion time. The moisture content and expressible drip in the perfused sample increased in a perfusion time-dependent manner. Low-field nuclear magnetic resonance (LF-NMR) measurements showed that1HT2relaxation time was prolonged in samples perfused for 30–60 s (p< 0.05). In1HT2-weighted images obtained using magnetic resonance imaging (MRI), high signal intensities were mainly observed in the dark muscle and segmental arteries and veins of samples perfused for 30–60 s, indicating the presence of free water that leaked from blood vessels. Therefore, water perfusion is an effective procedure for blood removal compared with conventional bleeding. Furthermore, in this study, water perfusion for 4–10 s was appropriate for both removing blood and preventing the quality deterioration induced by increased moisture content and expressible drip in horse mackerel muscle.