Extracellular matrix protein fibulin-2 is expressed in the embryonic endocardial cushion tissue and is a prominent component of valves in adult heart.
Extracellular matrix protein fibulin-2 is expressed in the embryonic endocardial cushion tissue and is a prominent component of valves in adult heart.
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细胞外基质蛋白 fibulin-2 在胚胎心内膜垫组织中表达，是成人心脏瓣膜的重要组成部分。
DOI：
10.1006/dbio.1995.1003
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ekblom,P
中科院分区：
文献类型：
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作者：
Zhang,HY;Chu,ML;Pan,TC;Sasaki,T;Timpl,R;Ekblom,P
Fibulins-1和Fibulins-2是新近发现的两种细胞外基质蛋白。它们在成体阶段的许多组织中被发现，但在培养的细胞和成体组织中注意到它们的表达模式的一些差异。Fibulin-1在胚胎发育过程中在内皮细胞垫组织（ECT）中表达特别好，因此我们比较了fibulin-1和-2在发育中小鼠心脏中的表达模式。免疫荧光法中使用了抗小鼠fibulin-1和-2抗体，并通过原位杂交用合成的寡核苷酸研究了mRNA的表达。fibulin-1和-2在内膜垫组织发育的所有阶段都有显著的共分布。发现Fibulin-2是胚胎ECT的比Fibulin-1更特异的标记物，Fibulin-1也在心肌中瞬时表达。免疫荧光显示，fibulins在成年期的心脏瓣膜中仍有表达。结果表明，在ECT的发展和维持心脏瓣膜的拉伸强度的两个fibulins的作用。
Fibulins-1 and -2 are two recently characterized extracellular matrix proteins of a novel protein family. They are found in many tissues in the adult stage, but several differences in their expression patterns were noted in cultured cells and adult tissues. Fibulin-1 is particularly well expressed in the endocardial cushion tissue (ECT) during embryogenesis and we therefore compared the expression patterns of fibulin-1 and -2 in the developing mouse heart. Antibodies against mouse fibulin-1 and -2 were used in immunofluorescence, and the expression of mRNAs were studied with synthetic oligonucleotides by in situ hybridization. A striking codistribution of fibulin-1 and -2 was seen at all stages of the development of the endocardial cushion tissue. Fibulin-2 was found to be a more specific marker for the embryonic ECT than fibulin-1, which was also transiently expressed in cardiac muscle. Immunofluorescence showed that the fibulins remained expressed in valves of the heart in the adult stage. The results suggest a role of the two fibulins in the development of the ECT and in the maintenance of the tensile strength of the cardiac valves.