Investigation of photobiomodulation potentiality by 635 and 809 nm lasers on human osteoblasts
Investigation of photobiomodulation potentiality by 635 and 809 nm lasers on human osteoblasts
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DOI：
10.1007/s10103-017-2153-6
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发表时间：
2017-04-01
影响因子：
2.1
通讯作者：
Gulsoy, Murat
中科院分区：
文献类型：
--
作者：
Ates, Gamze Bolukbasi;Can, Ayse Ak;Gulsoy, Murat
光生物调节（PBM）描述了通过应用低能量密度的红色或近红外激光/LED光实现的光诱导光化学反应。这种非侵入性和无痛的方法已被用于一些临床领域，但有争议的结果需要怀疑其有前途的和潜在的影响。在这项详细的体外研究中，用635和809 nm的二极管激光以0.5、1和2 J/cm（2）的能量密度照射成骨细胞。采用四甲基偶氮唑蓝（MTT）比色法、碱性磷酸酶（ALP）活性测定、茜素红染色、吖啶橙子/碘化丙啶染色、碱性磷酸酶（ALP）活性测定、逆转录聚合酶链反应（RT-PCR）检测I型胶原、ALPL和骨钙素的表达，评价细胞活力、增殖、骨形成和成骨分化。结果表明，所研究的能量剂量对成骨细胞的活力和增殖具有短暂的影响（激光照射后48小时）。同样，激光照射似乎对ALP活性没有任何影响。成骨细胞标志物的RT-PCR分析证实了这些结果。这项研究表明，在激光治疗成为临床水平上骨组织再生的假定应用之前，应在实验室中明确建立几个照射参数和方法的变化。
Photobiomodulation (PBM) describes light-induced photochemical reactions achieved by the application of red or near infrared lasers/LED light with low energy densities. This noninvasive and painless method has been used in some clinical areas but controversial outcomes demand a skeptical look for its promising and potential effects. In this detailed in vitro study, the osteoblast cells were irradiated with 635 and 809 nm diode lasers at energy densities of 0.5, 1, and 2 J/cm(2). Cell viability, proliferation, bone formation, and osteoblast differentiation were evaluated by methylthiazole tetrazolium (MTT) assay, Alamar Blue assay, acridine orange/propidium iodide staining, alkaline phosphatase (ALP) activity, Alizarin red staining, and reverse-transcription polymerase chain reaction (RT-PCR) to test the expression of collagen type I, ALPL, and osteocalcin. The results indicate that studied energy doses have a transient effect (48 h after laser irradiation) on the osteoblast viability and proliferation. Similarly, laser irradiation did not appear to have any effect on ALP activity. These results were confirmed by RT-PCR analysis of osteoblast markers. This study suggests that several irradiation parameters and variations in the methods should be clearly established in the laboratory before laser treatment becomes a postulated application for bone tissue regeneration in clinical level.