Epitranscriptomic Analysis of N6-methyladenosine in Infant Rhesus Macaques after Multiple Sevoflurane Anesthesia
Epitranscriptomic Analysis of N6-methyladenosine in Infant Rhesus Macaques after Multiple Sevoflurane Anesthesia
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DOI：
10.1016/j.neuroscience.2021.11.030
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发表时间：
2022-02-01
期刊：
影响因子：
3.3
通讯作者：
Jiang, Hong
中科院分区：
文献类型：
--
作者：
Chen, Xiao;Shi, Lingling;Jiang, Hong
迄今为止的临床研究表明，接触麻醉剂可能与神经发育缺陷有关。七氟醚是儿科患者最常用的全身麻醉剂。动物研究表明，产后多次接触七氟醚会导致大脑神经病理学变化和长期认知缺陷。然而，其根本机制仍有待阐明。在本研究中，通过甲基化 RNA 免疫沉淀测序 (MeRIP-Seq) 来获取幼年恒河猴前额皮质 RNA N6-甲基腺苷 (m(6)A) 的全基因组分析。七氟醚组的猕猴比对照组的猕猴有更多的 m(6)A 峰 (p
Clinical investigations to date have proposed the possibility that exposure to anesthetics is associated with neurodevelopmental deficits. Sevoflurane is the most commonly used general anesthetic in pediatric patients. Animal studies have demonstrated that multiple exposures to sevoflurane during the postnatal period resulted in neuropathological brain changes and long-term cognitive deficits. However, the underlying mechanisms remain to be clarified. In this study, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was performed to acquire genome-wide profiling of RNA N6-methyladenosine (m(6)A) in the prefrontal cortex of infant rhesus macaques. The macaques in the sevoflurane group had more m(6)A peaks than the macaques in the control group (p