Behavioural performance in three substrains of mouse strain 129
Behavioural performance in three substrains of mouse strain 129
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DOI：
10.1016/s0006-8993(97)00370-3
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发表时间：
1997-07-11
期刊：
影响因子：
2.9
通讯作者：
Holsboer, F
中科院分区：
文献类型：
--
作者：
Montkowski, A;Poettig, M;Holsboer, F
最近，人们提出了一种可能性，即在零突变小鼠中观察到的行为异常可能是由它们的遗传背景决定的，而不是由基因功能的丧失决定的，特别是当129小鼠品系被用作胚胎干细胞（ES）的供应商时。为了研究这一问题，我们在Morris水迷宫、开阔地迷宫、加迷宫和两项运动协调性测试中测试了3个129小鼠亚系（129/J、129/Ola、129/Sv-ter/+）和C57BL/6 （B6）。我们只在129/J亚型中发现了严重的行为缺陷。这些小鼠是白化病，携带粉红色眼睛稀释等位基因，在开放场地试验中评估的基础焦虑水平不同。他们在空间学习和记忆（Morris水迷宫测试）、Porsolt游泳测试（也测量学习和运动协调）中受到严重损害。然而，129/J亚株未被用于观察到行为异常的零突变小鼠的胚胎干细胞供体。相反，来自129/Ola和129/Sv-ter/+亚株的小鼠通常被用作胚胎干细胞的供应商。在包括Morris水迷宫试验在内的大部分试验中，这些小鼠表现正常。
Recently, the possibility has been raised that the behavioural abnormalities seen in null-mutant mice might be determined by their genetic background rather than by loss of gene function, especially when the 129 mouse strain is used as supplier for embryonic stem (ES) cells. To examine this issue we tested three 129 mouse substrains (129/J, 129/Ola, 129/Sv-ter/+) and C57BL/6 (B6) in the Morris water maze, the open field, the plus maze and two tests assessing motor co-ordination. We identified only for the 129/J substrain substantial behavioural deficits. These mice are albinos and carry the pink-eyed dilution allele and differed in their basal anxiety level as assessed in the open-field test. They were severely impaired in spatial learning and memory (Morris water maze test), in the Porsolt swim test, which also measures learning and in motor co-ordination. However, the 129/J substrain has not been used as ES cell donor in null-mutant mice where behavioural abnormalities were observed. Instead, mice from 129/Ola and 129/Sv-ter/+ substrains have been commonly used as suppliers for ES cells. These performed normally in most of the tests, including Morris water maze test.