An investigation of the nuclei of hülle cells of Aspergillus nidulans
An investigation of the nuclei of hülle cells of Aspergillus nidulans
复制标题
构巢曲霉壳细胞核的研究
DOI：
10.1590/s1415-47572002000400019
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发表时间：
2002
影响因子：
2.1
通讯作者：
I. R. Baracho
中科院分区：
文献类型：
--
作者：
M. F. Carvalho;M. Baracho;I. R. Baracho
曲霉菌属的某些种类的真菌在锁骨囊周围产生一种被称为壳细胞的细胞。为了便于对这些细胞的形成进行分析，获得了一株具有高脱壳细胞产量的中性曲霉菌株。这种菌株的Hulle细胞被允许在透析膜上生长，在那里可以观察到它们的细胞核。材料用Carnoy固定，用HCl水解，用Giemsa染色。在壳细胞形成阶段，观察到几个与菌丝和分生孢子核大小相当的细胞核。成熟的壳细胞形成约3.1 mm的大核。为了比较DNA的含量，分离出CH-89菌株的壳细胞和分生孢子进行DNA提取（Tuyl, 1977）。每个细胞的DNA含量平均是分生孢子DNA含量的17.2倍。
Certain species of fungi of the genus Aspergillus produce a type of cells around the cleistothecium that are called hulle cells. To facilitate the analysis of the formation of these cells, an Aspergillus nidulans strain with high hulle cell production was obtained. Hulle cells from this strain were allowed to grow on dialysis membranes, where it was possible to observe their nuclei. The material was fixed with Carnoy, hydrolyzed with HCl and stained with Giemsa. Several nuclei approximately equal in size to the nuclei of hyphae and conidia were observed during the phase of hulle cell formation. The formation of a macronucleus measuring approximately 3.1 mm was observed in mature hulle cells. To compare the DNA amount, hulle cells and conidia of the CH-89 strain were isolated and submitted to DNA extraction (Tuyl, 1977). The amount of DNA per cell was, on average, 17.2 times greater than the amount of DNA in the conidia.