Fluorescent protein complementation assays: new tools to study G protein-coupled receptor oligomerization and GPCR-mediated signaling.
Fluorescent protein complementation assays: new tools to study G protein-coupled receptor oligomerization and GPCR-mediated signaling.
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DOI：
10.1016/j.mce.2010.07.011
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发表时间：
2011-01-15
影响因子：
4.1
通讯作者：
Watts, Val J.
中科院分区：
文献类型：
--
作者：
Vidi, Pierre-Alexandre;Ejendal, Karin F. K.;Przybyla, Julie A.;Watts, Val J.
G蛋白偶联受体（GPCR）信号转导是通过多水平的蛋白质-蛋白质相互作用介导的。因此，相应的蛋白质复合物的表征是至关重要的GPCR介导的信号转导的基本理解。涉及GPCR的记录相互作用的数量正在迅速增长，了解这些复合物的功能意义显然是下一个挑战。新的实验方法，包括蛋白质互补测定（PCA）最近已被用来检查的组成，质膜靶向，和脱敏的蛋白质复合物参与GPCR信号。这些方法也有望更好地了解药物诱导的GPCR相互作用的影响。本文综述了荧光PCA在GPCR信号研究中的应用。还介绍了PCA在高内容屏幕中的潜在应用。非荧光PCA技术，以及三元和四元蛋白质复合物的检测组合测定进行了简要讨论。
G protein-coupled receptor (GPCR) signaling is mediated by protein-protein interactions at multiple levels. The characterization of the corresponding protein complexes is therefore paramount to the basic understanding of GPCR-mediated signal transduction. The number of documented interactions involving GPCRs is rapidly growing, and appreciating the functional significance of these complexes is clearly the next challenge. New experimental approaches including protein complementation assays (PCAs) have recently been used to examine the composition, plasma membrane targeting, and desensitization of protein complexes involved in GPCR signaling. These methods also hold promise for better understanding of drug-induced effects on GPCR interactions. This review focuses on the application of fluorescent PCAs for the study of GPCR signaling. Potential applications of PCAs in high-content screens are also presented. Non-fluorescent PCA techniques as well as combined assays for the detection of ternary and quaternary protein complexes are briefly discussed.
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影响因子：
4.7
作者：
Canals, M;Burgueño, J;Franco, R
通讯作者：
Franco, R
影响因子：
4.8
作者：
Armstrong, D;Strange, PG
通讯作者：
Strange, PG
影响因子：
3.9
作者：
Eglen, R. M.
通讯作者：
Eglen, R. M.
影响因子：
4.7
作者：
Ferré S;Goldberg SR;Lluis C;Franco R
通讯作者：
Franco R
DOI：
10.1046/j.1432-1033.2003.03773.x
发表时间：
2003-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Gazi, L;López-Giménez, JF;Strange, PG
通讯作者：
Strange, PG