Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen.
Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen.
复制标题
测量单个组织样本中脂质介质、蛋白质和信使 RNA 的方法。
DOI：
10.1016/j.ab.2014.10.004
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发表时间：
2015
影响因子：
2.9
通讯作者：
O'Connor,JPatrick
中科院分区：
文献类型：
--
作者：
Cottrell,JessicaA;Lin,Hsuan-Ni;O'Connor,JPatrick
This article describes a new method for extracting RNA, protein, and lipid mediators from a single tissue specimen. Specifically, mouse bone fracture callus specimens were extracted into a single solution that was processed using three different procedures to measure messenger RNA (mRNA) levels by reverse transcription–quantitative polymerase chain reaction (RTqPCR), cytokines and growth factors using an xMAP method, and lipid mediators by liquid chromatography–tandem mass spectrometry (LC–MS/MS). This method has several advantages because it decreases the number of animals necessary for experimentation, allows division of the sample from a homogeneous mixture that reduces sample variability, and uses a solution that protects the integrity of the macromolecules during storage.
