Correlation between complementation group for immortality and the cellular distribution of mortalin.
Correlation between complementation group for immortality and the cellular distribution of mortalin.
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DOI：
10.1006/excr.1995.1013
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发表时间：
1995
影响因子：
3.7
通讯作者：
R. Wadhwa;O. Pereira-smith;R. Reddel;Yoshikazu Sugimoto;Y. Mitsui;S. Kaul
中科院分区：
文献类型：
--
作者：
R. Wadhwa;O. Pereira-smith;R. Reddel;Yoshikazu Sugimoto;Y. Mitsui;S. Kaul
利用人和小鼠细胞的细胞融合实验已经证明了细胞衰老对永生表型的优势。Mortalin是小鼠hsp 70蛋白家族的一个新的66-kDa成员，由于其在死亡细胞中的特征性胞质分布，最近已被鉴定为死亡表型的标志物。在这里，我们报告了21人细胞系的死亡蛋白免疫染色观察。这些细胞系先前已通过体细胞杂交分析分配到四个互补组（A、B、C和D）中的一个（18个细胞系）或一个以上（3个细胞系）用于永生化。观察到四种模式的死亡蛋白免疫染色：颗粒-单核帽，颗粒-梯度从核到细胞膜，颗粒-单核弓，和纤维-核周。在17的18个细胞系分配到一个单一的互补组，死亡蛋白染色对应的互补组。在先前分配到多个互补组的三个细胞系中的两个中，死亡蛋白染色对应于分配的组之一。然而，两个细胞系，表现出染色模式，不匹配其分配的互补组。死亡蛋白的细胞分布与互补基团之间的相关性的基础目前仍不清楚。然而，这些数据（i）表明，细胞内分布的死亡蛋白可以用来区分死亡和不朽的细胞，确认死亡蛋白与衰老的关联;（ii）提供支持性证据，至少有四种不同的途径的永生化在人类细胞的存在;和（iii）表明，死亡蛋白参与的过程，导致永生化。
The dominance of cellular senescence over the immortal phenotype has been demonstrated by cell fusion experiments utilizing human and mouse cells. Mortalin, a novel 66-kDa member of the murine hsp70 family of proteins, has recently been identified as a marker of the mortal phenotype by virtue of its characteristic cytosolic distribution in mortal cells. Here we report the mortalin immunostaining observations on 21 human cell lines. These cell lines have previously been assigned by somatic cell hybridization analysis to one (18 lines) or more than one (3 lines) of the four complementation groups (A, B, C, and D) for immortalization. Four patterns of mortalin immunostaining were observed: granular-juxtanuclear cap, granular-gradient from nuclear to cell membrane, granular-juxtanuclear arch, and fibrous-perinuclear. In 17 of 18 cell lines assigned to a single complementation group, the mortalin staining corresponded with the complementation group. In two of the three cell lines previously assigned to multiple complementation groups, the mortalin staining corresponded to one of the assigned groups. Two cell lines, however, exhibited staining patterns which did not match to their assigned complementation groups. The basis of correlation between cellular distribution of mortalin and the complementation group remains unclear at present. However, the data (i) suggest that the intracellular distribution of mortalin can be used to distinguish mortal and immortal cells, confirming the association of mortalin with senescence; (ii) provide supportive evidence for the existence of at least four different pathways of immortalization in human cells; and (iii) indicate that mortalin is involved in processes that result in immortalization.