Conspecific coprophagy stimulates normal development in a germ-free model invertebrate
Conspecific coprophagy stimulates normal development in a germ-free model invertebrate
复制标题
DOI：
10.7717/peerj.6914
复制
发表时间：
2019-05-13
期刊：
影响因子：
2.7
通讯作者：
Sabree, Zakee L.
中科院分区：
文献类型：
--
作者：
Jahnes, Benjamin C.;Herrmann, Madeline;Sabree, Zakee L.
驻留在动物胃组织内和动物胃组织上的微生物聚集体有助于各种宿主有益过程，包括饮食可及性和营养供应，我们试图研究代际和社区获得性肠道细菌在多大程度上影响易处理的无菌（GF）无脊椎动物模型系统的发育。食粪是蟑螂和白蚁的一种常见行为，它提供了获得营养物质的途径，也是给青少年接种有益肠道细菌的主要手段。通过干扰这种获取肠道细菌的方式在美洲大蠊（Periplaneta americana）中测试了这一假设，这导致GF昆虫相对于野生型（WT）美洲大蠊表现出延长的生长速率和肠道组织畸形。通过消耗在非无菌条件下饲养的同种和同胞的粪便（粪便），GF美洲原住民的常规化导致美洲原住民肠道组织被多种微生物群落定殖，并且WT水平生长和后肠组织发育表型显着恢复（p < 0.05）。这些数据表明，通过将有益的肠道细菌引入美洲原教旨主义者，食粪对于正常的肠道组织和生物体发育是必不可少的，并且GF美洲原教旨主义者模型系统是用于检查肠道细菌如何影响宿主结果的有用系统。
Microbial assemblages residing within and on animal gastric tissues contribute to various host beneficial processes that include diet accessibility and nutrient provisioning, and we sought to examine the degree to which intergenerational and community-acquired gut bacteria impact development in a tractable germ-free (GF) invertebrate model system. Coprophagy is a common behavior in cockroaches and termites that provides access to both nutrients and the primary means by which juveniles are inoculated with beneficial gut bacteria. This hypothesis was tested in the American cockroach (Periplaneta americana) by interfering with this means of acquiring gut bacteria, which resulted in GF insects that exhibited prolonged growth rates and gut tissue dysmorphias relative to wild-type (WT) P. americana. Conventionalization of GF P. americana via consumption of frass (feces) from conspecifics and siblings reared under non-sterile conditions resulted in colonization of P. americana gut tissues by a diverse microbial community and a significant (p < 0.05) recovery of WT level growth and hindgut tissue development phenotypes. These data suggest that coprophagy is essential for normal gut tissue and organismal development by introducing beneficial gut bacteria to P. americana, and that the GF P. americana model system is a useful system for examining how gut bacteria impact host outcomes.