Recombinant erythropoietin down-regulates IL-6 and CXCR4 genes in TNF-α-treated primary cultures of human microvascular endothelial cells -: Implications of multiple sclerosis
Recombinant erythropoietin down-regulates IL-6 and CXCR4 genes in TNF-α-treated primary cultures of human microvascular endothelial cells -: Implications of multiple sclerosis
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DOI：
10.1385/jmn:25:2:183
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Konduru, SS
中科院分区：
文献类型：
--
作者：
Avasarala, JR;Konduru, SS
在多发性硬化症（MS）中，血脑屏障的破坏可能导致脑中新的钆增强病变形成并导致急性复发。目前MS急性复发的治疗选择有限。研究了重组促红细胞生成素（rEPO）对TNF-α处理的人脑微血管内皮细胞中细胞因子基因表达的影响。细胞为对照（未处理），暴露于TNF-α或TNF-α/rEPO 6或24小时。在研究的96个基因中，与单独TNF-α相比，TNF-α/rEPO处理6 h后，白细胞介素-6（IL-6）、IL-1 β、CXCR 4和IL-1 α基因下调。24 h时IL-6和CXCR 4基因表达分别为4.24和2.98。定量RT-PCR分析显示IL-6和CXCR 4基因分别下调3.86和1.9。我们的研究结果表明，进一步的研究是必要的，以评估使用促红细胞生成素在最大限度地减少急性复发MS。
In multiple sclerosis (MS), disruption of the blood-brain barrier might lead to new gadolinium-enhanced lesion formation in the brain and cause acute relapses. Current therapeutic options for acute relapses in MS are limited. The effect of recombinant erythropoietin (rEPO) on cytokine gene expression in TNF-alpha-treated human brain microvascular endothelial cells was studied. The cells were controls (untreated), exposed for either 6 or 24 h to TNF-alpha or TNF-alpha/rEPO. Of the 96 genes studied, interleukin-6 (IL-6), IL-1 beta, CXCR4, and IL-1 alpha genes were down-regulated when treated with TNF-alpha/rEPO for 6 h as compared with TNF-alpha alone. At 24 h, IL-6 and CXCR4 gene expression was 4.24 and 2.98, respectively. Quantitative RT-PCR analysis showed down-regulation by 3.86 and 1.9 for IL-6 and CXCR4 genes, respectively. Our findings suggest that further studies are warranted to evaluate the use of EPO in minimizing acute relapses in MS.